Anti-α2C-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-α2C-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Mukherjee K, Xue L, Planutis A, Gnanapragasam M, Chess A, Bieker J. EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis. elife. 2021;10: pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 4), kidney (lanes 2 and 5) and brain (lanes 3 and 6) membranes: - 1-3. Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022), (1:200).4-6. Anti-α2C-Adrenergic Receptor (extracellular) Antibody, preincubated with α2C-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR022).

image 2 :

Expression of α2C-Adrenoreceptor in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded sections using Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022), (1:100). α2C-Adrenoreceptor is expressed in both proximal (PT) and distal (DT) renal tubules and in the collecting ducts (CD). Hematoxilin is used as the counterstain.

product information

CAT :

AAR-022

SKU :

AAR-022-CF_0.2 ml

Product Name :

Anti-α2C-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22086

Applications :

IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR022

Homology :

Rat, mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RRPDGAAYPQ*SGLNDE, corresponding to amino acid residues 192-207 of rat α2C-adrenoceptor with replacement of cysteine 202 (C202) with serine (*S)

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2.The α2C-adrenoceptor subtype was identified by binding studies in the opossum kidney and in cell lines from this organ (OK cells)3,4.Surprisingly, the α2C-adrenoceptor subtype seems not to play a major role in cardiovascular regulation or the other classical effects of α2-adrenergic receptors5. Some researchers have suggested that the α2C-adrenoceptor subtype may play a role in modulating motor behaviour and perhaps in memory processes6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Alpha-2C adrenoceptor, Alpha-2C adrenoreceptor, Alpha-2CAR, ADA2C, ADRA2C

Short Description :

A Rabbit Polyclonal Antibody to α2C-Adrenergic Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat α2C-adrenoceptor. Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022) can be used for western blot and immunohistochemistry applications. It has been designed to recognize α2C-adrenoceptor from rat, mouse and human samples.

Negative Control :

BLP-AR022

Positive Control :

Synonyms :

Alpha-2C adrenoceptor, Alpha-2C adrenoreceptor, Alpha-2CAR, ADA2C, ADRA2C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRA2C

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage