Anti-α2B-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-α2B-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Mukherjee K, Xue L, Planutis A, Gnanapragasam M, Chess A, Bieker J. EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis. elife. 2021;10: pubmed publisher
Wang Z, Rodrigues A, Messi M, Delbono O. Aging Blunts Sympathetic Neuron Regulation of Motoneurons Synaptic Vesicle Release Mediated by β1- and α2B-Adrenergic Receptors in Geriatric Mice. J Gerontol A Biol Sci Med Sci. 2020;75:1473-1480 pubmed publisher
Hamlett E, Ledreux A, Gilmore A, Vazey E, Aston Jones G, Boger H, et al. Inhibitory designer receptors aggravate memory loss in a mouse model of down syndrome. Neurobiol Dis. 2020;134:104616 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 5), lung (lanes 2 and 6), liver (lanes 3 and 7) lysates and rat skeletal muscle membranes (lanes 4 and 8): - 1-4. Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:200).5-8. Anti-α2B-Adrenergic Receptor (extracellular) Antibody, preincubated with α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021).

image 2 :

Expression of α2B-Adrenoreceptor in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:100). α2B-Adrenoreceptor is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of RAMP1 in rat PC12 cells - Cell surface detection ofRAMP1 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells usingAnti-RAMP1 (extracellular) Antibody(#ARR-021) (1:50)(red). B. Live view of cells.

product information

CAT :

AAR-021

SKU :

AAR-021-CF_0.2 ml

Product Name :

Anti-α2B-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19328

Applications :

IFC IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR021

Homology :

Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical; human - 10/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat α2B-adrenoceptor with replacement of cysteine 169 (C169) with serine (*S)

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. The α2B-adrenoceptor has a distinct pattern of expression within the brain, liver lung and kidney, and recent studies using the knock out mouse system have shown that disruption of this receptor indeed affects mouse viability3, blood pressure responses to α2-adrenoceptor agonists3 and the hypertensive response to salt loading4. Like the α2A-adrenoceptor subtype, the α2B-adrenoceptor undergoes short term agonist promoted desensitization5. This desensitization is due to the phosphorylation of the receptor by G-protein coupled receptor kinases (GRKs)6 which ultimately promotes uncoupling of the receptor from the G-protein subunit7.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B

Short Description :

A Rabbit Polyclonal Antibody to α2B-Adrenergic Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat α2B-adrenoceptor. Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2B-adrenoceptor from mouse, rat, and human samples.

Negative Control :

BLP-AR021

Positive Control :

Synonyms :

Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRA2B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage