antibody

Anti-α2A-Adrenergic Receptor (extracellular) Antibody

AAR-020

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

AAR-020-CF_0.2 ml

Anti-α2A-Adrenergic Receptor (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P22909

IF IFC IHC WB

Human Rat Mouse

Rabbit

BLP-AR020

Mouse - identical; human - 13/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat α2A-adrenoceptor

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine; rat and mouse α2A displays less affinity compared to human3,4.

IF IFC IHC WB

Alomone Labs

Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A

A Rabbit Polyclonal Antibody to α2A-Adrenergic Receptor

Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2A-adrenoceptor from mouse, rat, and human samples.

BLP-AR020

NA

Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADRA2A

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

IHC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:250

1:200