Anti-α1B-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-α1B-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Leo M, Schmitt L, Jastrow H, Thomale J, Kleinschnitz C, Hagenacker T. Cisplatin alters the function and expression of N-type voltage-gated calcium channels in the absence of morphological damage of sensory neurons. Mol Pain. 2017;13:1744806917746565 pubmed publisher
Masukawa D, Koga M, Sezaki A, Nakao Y, Kamikubo Y, Hashimoto T, et al. L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor. JCI Insight. 2017;2: pubmed publisher

image

image 1 :

Western blot analysis of α1B-Adrenoceptor in rat brain (lanes 1 and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6) lysates: - 1,2,5. Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:200).3,4,6. Anti-α1B-Adrenergic Receptor (extracellular) Antibody, preincubated with α1B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR018).

image 2 :

Expression of α1B-Adrenoceptor in rat cerebellum - Immunohistochemical staining of frozen section of rat cerebellum using Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:100). α1B-Adrenoceptor (red) is detected in Purkinje cells. Hoechst 33342 was used as the counterstain.

image 3 :

Expression of ?1B-Adrenoceptor in GH3 cells - Cell surface detection of?1B-Adrenoceptor in living GH3 cells with Anti-?1B-Adrenergic Receptor(extracellular) Antibody(#AAR-018) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the DNA dye Hoechst 33342 (blue).

product information

CAT :

AAR-018

SKU :

AAR-018-CF_0.2 ml

Product Name :

Anti-α1B-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35368

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR018

Homology :

Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4 α1B-Adrenergic receptor populations are greatest in the spleen, kidney, cerebellum, and fetal brain.5α1B-Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Alpha-1B adrenoceptor, ADA1B, ADRA1B

Short Description :

A Rabbit Polyclonal Antibody to α1B-Adrenergic Receptor

Long Description :

Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018) is a highly specific antibody directed against an extracellular epitope of the human α1B-adrenoceptor. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize α1B-adrenoceptor from human, rat and mouse samples.

Negative Control :

BLP-AR018

Positive Control :

Synonyms :

Alpha-1B adrenoceptor, ADA1B, ADRA1B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRA1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage