Anti-β3-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-β3-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Saunders S, Hutchinson D, Britton F, Liu L, Markus I, Sandow S, et al. Effect of β1 /β2 -adrenoceptor blockade on β3 -adrenoceptor activity in the rat cremaster muscle artery. Br J Pharmacol. 2021;178:1789-1804 pubmed publisher
Silva I, Magalhães Cardoso M, Ferreirinha F, Moreira S, Costa A, Silva D, et al. β3 Adrenoceptor-induced cholinergic inhibition in human and rat urinary bladders involves the exchange protein directly activated by cyclic AMP 1 favoring adenosine release. Br J Pharmacol. 2020;177:1589-1608 pubmed publisher
Briot J, Mailhot O, Bourdin B, Tétreault M, Najmanovich R, Parent L. A three-way inter-molecular network accounts for the CaVα2δ1-induced functional modulation of the pore-forming CaV1.2 subunit. J Biol Chem. 2018;293:7176-7188 pubmed publisher
Bourdin B, Briot J, Tétreault M, Sauve R, Parent L. Negatively charged residues in the first extracellular loop of the L-type CaV1.2 channel anchor the interaction with the CaV?2?1 auxiliary subunit. J Biol Chem. 2017;292:17236-17249 pubmed publisher
Segura E, Bourdin B, Tétreault M, Briot J, Allen B, Mayer G, et al. Proteolytic cleavage of the hydrophobic domain in the CaV?2?1 subunit improves assembly and activity of cardiac CaV1.2 channels. J Biol Chem. 2017;292:11109-11124 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,3. Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:200).2,4. Anti-β3-Adrenergic Receptor (extracellular) Antibody, preincubated with β3-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR017).

image 2 :

Expression of β3-Adrenoceptor in rat hippocampus - Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:100), (green). β3-Adrenoceptor is strongly expressed in the hilus (H) and in the outer molecular layer (arrows). The distribution overlaps the entire layers, but is not restricted to nerve cells (stained red with mouse anti-Parvalbumin). DAPI is used as the counterstain (blue).

image 3 :

Expression of ?3-Adrenoceptor in rat hippocampus - Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-?3-Adrenergic Receptor (extracellular)Antibody (#AAR-017)(1:100) (green). ?3-Adrenoceptor is strongly expressed in the hilus (H) and in the outer molecular layer (arrows). The distribution overlaps the entire layers but is not restricted to nerve cells (stained red with mouse anti-Parvalbumin). DAPI is used as the counterstain (blue).

product information

CAT :

AAR-017

SKU :

AAR-017-CF_0.2 ml

Product Name :

Anti-β3-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25962

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR017

Homology :

Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S)

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 The β3-adrenoceptor is insensitive to the commonly used β-antagonists and has often been referred to as the 'atypical' β-adrenoceptor. Mouse β3-adrenoceptor has two known splice variants; isoforms β3a-adrenoceptor and β3b-adrenoceptor. Both isoforms are expressed in white and brown adipose tissues.3 The β3b-adrenoceptor is highly expressed in brain.The β3- adrenoceptor has been found to play a key role in the lipolytic action of catecholamines.4

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR

Short Description :

A Rabbit Polyclonal Antibody to β3-Adrenergic Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse β3-adrenoceptor. Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of the β3AR from mouse, rat and human samples.

Negative Control :

BLP-AR017

Positive Control :

Synonyms :

Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRB3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage