Anti-β2-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-β2-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Arora V, Morado Urbina C, Gwak Y, Parker R, Kittel C, Muñoz Islas E, et al. Systemic administration of a β2-adrenergic receptor agonist reduces mechanical allodynia and suppresses the immune response to surgery in a rat model of persistent post-incisional hypersensitivity. Mol Pain. 2021;17:1744806921997206 pubmed publisher
Lee M, Mueller A, Moore T. Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field. Front Neuroanat. 2020;14:574130 pubmed publisher
Evans A, Ardestani P, Yi B, Park H, Lam R, Shamloo M. Beta-adrenergic receptor antagonism is proinflammatory and exacerbates neuroinflammation in a mouse model of Alzheimer's Disease. Neurobiol Dis. 2020;146:105089 pubmed publisher
Silva I, Magalhães Cardoso M, Ferreirinha F, Moreira S, Costa A, Silva D, et al. β3 Adrenoceptor-induced cholinergic inhibition in human and rat urinary bladders involves the exchange protein directly activated by cyclic AMP 1 favoring adenosine release. Br J Pharmacol. 2020;177:1589-1608 pubmed publisher

image

image 1 :

Western blot analysis of rat heart (lanes 1 and 3) and lung (lanes 2 and 4) lysates: - 1,3. Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:200).2,4. Anti-β2-Adrenergic Receptor (extracellular) Antibody, preincubated with β2-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR016).

image 2 :

Expression of β2-Adrenoceptor in rat lung - Immunohistochemical staining of paraffin-embedded section of rat lung using Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:100). β2-Adrenoceptor is expressed in the respiratory epithelium. Hematoxilin is used as the counterstain.

image 3 :

Expression of?2-Adrenoceptor in rat lung - Immunohistochemical staining of paraffin-embedded section of rat lung using Anti-?2-Adrenergic Receptor (extracellular)Antibody (#AAR-016) (1:100). ?2-Adrenoceptor is expressed in the respiratory epithelium. Hematoxilin is used as the counterstain.

product information

CAT :

AAR-016

SKU :

AAR-016-CF_0.2 ml

Product Name :

Anti-β2-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18762

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR016

Homology :

Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).5

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Short Description :

A Rabbit Polyclonal Antibody to ?2-Adrenergic Receptor

Long Description :

Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2AR from mouse, rat, and human samples.

Negative Control :

BLP-AR016

Positive Control :

Synonyms :

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage