Anti-β2-Adrenoceptor (extracellular)-PE Antibody | Alomone Labs AAR-016-PE product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-β2-Adrenoceptor (extracellular)-PE Antibody

catalog :

AAR-016-PE

clonality :

polyclonal

host :

domestic rabbit

conjugate :

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Lee M, Mueller A, Moore T. Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field. Front Neuroanat. 2020;14:574130 pubmed publisher

image

image 1 :

Cell surface detection of β2-Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cells: Cell surface detection of β2-Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-PE. Green line: Cells + Anti-β2-Adrenergic Receptor (extracellular)-PE Antibody (#AAR-016-PE), 2.5µg.

product information

CAT :

AAR-016-PE

SKU :

AAR-016-PE_50 mcl

Product Name :

Anti-β2-Adrenoceptor (extracellular)-PE Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18762

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR016

Homology :

Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

R-Phycoerythrin (R-PE).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).5

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

β2-Adrenergic receptor, Beta-2 Adrenergic receptor, Beta-2 Adrenoceptor, B2AR, ADRB2

Short Description :

A Rabbit Polyclonal Antibody to β2-Adrenergic Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)

Long Description :

Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ADRB2 from mouse, rat, and human samples. Anti-β2-Adrenergic Receptor (extracellular)-PE Antibody (#AAR-016-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-PE

Positive Control :

Synonyms :

β2-Adrenergic receptor, Beta-2 Adrenergic receptor, Beta-2 Adrenoceptor, B2AR, ADRB2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRB2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-016), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage