antibody

Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody

AAR-016-F

polyclonal

domestic rabbit

FITC

NA

human, mouse, rat

AAR-016-F_50 mcl

Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

P18762

FC LCI

Human Rat Mouse

Rabbit

BLP-AR016

Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2

G-protein coupled receptors (GPCRs) comprise the largest protein superfamily in mammalian genomes. Signal transduction by GPCRs is fundamental for most physiological processes, spanning from vision, smell and taste to neurological, cardiovascular, endocrine, and reproductive functions, thus, making the GPCR superfamily a major target for therapeutic intervention.The β2-adrenergic receptor (ADRB2) is comprised of seven transmembrane α-helices, connected by three extracellular loops and three intracellular loops. The extracellular part, responsible for ligand binding, also includes the N-terminus. The intracellular C-terminal interacts with G-proteins, arrestins and other downstream effectors1.Unlike some GPCRs, ADRB2 activates more than one G-protein and signals through at least one known G-protein-independent pathway, arrestin. In addition, there is a rich diversity of available ligands for ADRB2. These ligands are often characterized as inverse agonists that suppress basal activity, full agonists that maximally activate the receptor, partial agonists that produce submaximal activity even at saturating concentrations, and neutral antagonists that occupy the orthosteric binding site but do not affect basal activity2.ADRB2 located on the surface of cardiomyocytes, mediates distinct effects on cardiac function and the development of heart failure by regulating production of the secondary messenger cyclic adenosine monophosphate (cAMP). In cardiomyocytes from healthy adult rats and mice, spatially confined ADRB2 induces cAMP signals that are localized exclusively to the deep transverse tubules. In contrary, in cardiomyocytes derived from a rat model of chronic heart failure, ADRB2 is redistributed from the deep transverse tubules to the cell crest, which leads to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of ADRB2 in heart failure might contribute to the failing myocardial phenotype3.

FC LCI

Alomone Labs

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

A Rabbit Polyclonal Antibody to β2-Adrenergic Receptor Conjugated to the Fluorescent Dye FITC

Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2AR from mouse, rat, and human samples. Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-016-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

RIC-001-F

NA

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADRB2

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

NA

Western blot analysis (unlabeled antibody, #AAR-016), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information