Anti-β2-Adrenergic Receptor (extracellular)-APC Antibody | Alomone Labs AAR-016-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-β2-Adrenergic Receptor (extracellular)-APC Antibody

catalog :

AAR-016-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of β 2 -Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cell line:

image 2 :

Cell surface detection of β 2 -Adrenergic Receptor by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:

product information

CAT :

AAR-016-APC

SKU :

AAR-016-APC_15 mcl

Product Name :

Anti-β2-Adrenergic Receptor (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18762

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR016

Homology :

Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.

Formulation :

Lyophilized Powder.

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).5

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Short Description :

A Rabbit Polyclonal Antibody to β2-Adrenergic Receptor conjugated to Allophycocyanin (APC) fluorophore.

Long Description :

Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize B2AR from mouse, rat, and human samples. Anti-β2-Adrenergic Receptor (extracellular)-APC Antibody (#AAR-016-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-APC

Synonyms :

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

H- Human, M- Mouse, R- Rat

AB Specifictiy :

ADRB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Purity :

Affinity purified on immobilized antigen.

Negative control name :

GLP1R (extracellular) Blocking Peptide

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-016), and direct flow cytometry (labeled antibody).

more info or order :

Alomone Labs product webpage