Anti-α1A-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-α1A-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Lee M, Mueller A, Moore T. Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field. Front Neuroanat. 2020;14:574130 pubmed publisher
Kohro Y, Matsuda T, Yoshihara K, Kohno K, Koga K, Katsuragi R, et al. Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity. Nat Neurosci. 2020;23:1376-1387 pubmed publisher
Newton J, Suman S, Akinfiresoye L, Datta K, Lovinger D, N GOUEMO P. Alcohol withdrawal upregulates mRNA encoding for CaV2.1-?1 subunit in the rat inferior colliculus. Alcohol. 2018;66:21-26 pubmed publisher
Hill B, Dalton R, Joseph B, Thakali K, Rusch N. 17?-estradiol reduces Cav 1.2 channel abundance and attenuates Ca2+ -dependent contractions in coronary arteries. Pharmacol Res Perspect. 2017;5: pubmed publisher

image

image 1 :

Western blot analysis of Human PC3 prostate carcinoma cell line lysate (lanes 1 and 3) and rat brain membrane (lanes 2 and 4): - 1,2. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:200).3,4. Anti-α1A-Adrenergic Receptor (extracellular) Antibody, preincubated with α1A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR015).

image 2 :

Expression of α1A-Adrenergic receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:100). A. α1A-adrenoceptor (green) appears in fibers of Bergmann glia (triangle points at an example). B. S100β (red), a marker of Bergmann glia, is stained in the same section. C. Merge of the images demonstrates expression of α1A-adrenoceptor in fibers of Bergmann glia. α1A-adrenoceptor is also expressed in cells in the molecular layer that are s100β negative (arrow points at an example). DAPI is used as the counterstain (blue).

image 3 :

Expression of ?1A-Adrenergic receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-?1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015) (1:100). A. ?1A-adrenoceptor (green) appears in fibers of Bergmann glia (triangle points at an example). B. S100? (red) a marker of Bergmann glia is stained in the same section. C. Merge of the images demonstrates expression of ?1A-adrenoceptor in fibers of Bergmann glia. ?1A-adrenoceptor is also expressed in cells in the molecular layer that are s100?negative (arrow points at an example). DAPI is used as the counterstain (blue).

product information

CAT :

AAR-015

SKU :

AAR-015-CF_0.2 ml

Product Name :

Anti-α1A-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35348

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR015

Homology :

Human, rat, mouse, bovine - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human α1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S)

Product Page - Scientific background :

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4The α1A-ARs have at least nine known alternative spliced isoforms. They are the most abundant adrenergic receptor in the brain, and are considered postsynaptic receptors. α1A-ARs are also expressed in the heart, liver and prostate.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, α1A-AR, ADRA1A

Short Description :

A Rabbit Polyclonal Antibody to ?1A-Adrenergic Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human α1A-adrenergic receptor. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α1A-adrenoceptor from human, rat, and mouse samples.

Negative Control :

BLP-AR015

Positive Control :

Synonyms :

Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, α1A-AR, ADRA1A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRA1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage