Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody | Alomone Labs AAR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody

catalog :

AAR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Jagannath A, Varga N, Dallmann R, Rando G, Gosselin P, Ebrahimjee F, et al. Adenosine integrates light and sleep signalling for the regulation of circadian timing in mice. Nat Commun. 2021;12:2113 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200). 4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).

image 2 :

Expression of Renin Receptor in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Renin Receptor immunoreactivity (green) appears in neuronal profiles in the purkinje cell layer (arrows). B. Pre-incubation of the antibody with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (BLP-AR014), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AAR-014

SKU :

AAR-014-CF_0.2 ml

Product Name :

Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9CYN9

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR014

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor

Product Page - Scientific background :

The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin. Renin is an aspartyl protease that cleaves angiotensinogen into angiotensin I, the rate-limiting reaction in the cascade generating angiotensin, a peptide hormone that is involved in blood pressure regulation. Renin binding by (P)RR affects important biological systems and pathways that are involved in cell cycle regulation, autophagy, acid-base balance, energy metabolism, embryonic development, T cell homeostasis, water balance, blood pressure regulation, cardiac remodeling and maintenance of podocyte structure1.In addition, (P)RR has been reported to contribute to the pathogenesis of diseases such as fibrosis, hypertension, pre-eclampsia, diabetic microangiopathy, acute kidney injury, cardiovascular disease, cancer and obesity1.The receptor is widely distributed in organs throughout the body, including the kidneys, heart, brain, eyes, placenta, and the immune system1.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2

Short Description :

A Rabbit Polyclonal Antibody to Renin Receptor

Long Description :

Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize ATP6AP2 from rat, mouse, and human samples. This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).

Negative Control :

BLP-AR014

Positive Control :

Synonyms :

Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP6AP2

AB Specifictiy :

This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage