Anti-Angiotensin-(1-7) Mas Receptor Antibody | Alomone Labs AAR-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Angiotensin-(1-7) Mas Receptor Antibody

catalog :

AAR-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:250; loading ...; fig 1b	Hammer A, Yang G, Friedrich J, Kovacs A, Lee D, Grave K, et al. Role of the receptor Mas in macrophage-mediated inflammation in vivo. Proc Natl Acad Sci U S A. 2016;113:14109-14114 pubmed
	Vieira Rocha M, Rodríguez Rodríguez P, Ferreira Duarte M, Faria M, Sousa J, Morato M, et al. Fetal Undernutrition Modifies Vascular RAS Balance Enhancing Oxidative Damage and Contributing to Remodeling. Int J Mol Sci. 2022;23: pubmed publisher
	Cao X, Shi T, Zhang C, Jin W, Song L, Zhang Y, et al. ACE2 pathway regulates thermogenesis and energy metabolism. elife. 2022;11: pubmed publisher
	Aparicio Prieto M, Rodr xed guez Gallego M, Valdivia Palac xed n A, Franco Iriarte Y, Herv xe1 s Barbara G, Echevarr xed a Orella E, et al. Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl. 2022;24:139-146 pubmed publisher
	Berenyiova A, Bernatova I, Zemancikova A, Drobna M, Cebova M, Golas S, et al. Vascular Effects of Low-Dose ACE2 Inhibitor MLN-4760-Benefit or Detriment in Essential Hypertension?. Biomedicines. 2021;10: pubmed publisher
	Dos Reis Costa D, Silveira A, Campos G, N xf3 brega N, de Ara xfa jo N, de Figueiredo Borges L, et al. High-Carbohydrate Diet Enhanced the Anticontractile Effect of Perivascular Adipose Tissue Through Activation of Renin-Angiotensin System. Front Physiol. 2020;11:628101 pubmed publisher
	Pedrosa M, Valenzuela R, Garrido Gil P, Labandeira C, Navarro G, Franco R, et al. Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19. Clin Sci (Lond). 2021;135:465-481 pubmed publisher
	Silva C, Correa A, Kushmerick C, Sharma N, Patel K, de Almeida J, et al. Angiotensin-converting enzyme 2 activator, DIZE in the basolateral amygdala attenuates the tachycardic response to acute stress by modulating glutamatergic tone. Neuropeptides. 2020;83:102076 pubmed publisher
	Raupp D, Fernandes R, Antunes K, Perin F, Rigatto K. Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis. Peptides. 2020;133:170384 pubmed publisher
	Evans C, Miners J, Piva G, Willis C, Heard D, Kidd E, et al. ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease. Acta Neuropathol. 2020;139:485-502 pubmed publisher
	Beitia M, Solano Iturri J, Errarte P, Sanz B, Perez I, Etxezarraga M, et al. Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence. Int J Med Sci. 2019;16:813-821 pubmed publisher
	Foulquier S, Caolo V, Swennen G, Milanova I, Reinhold S, Recarti C, et al. The role of receptor MAS in microglia-driven retinal vascular development. Angiogenesis. 2019;22:481-489 pubmed publisher
	Xiao H, Zhao L, Yang J, Tong N, An L, Liu Q, et al. Imbalance of angiotensin-converting enzymes affects myocardial apoptosis during cardiac arrest induced by acute pulmonary embolism in a porcine model. Int J Mol Med. 2019;43:1575-1584 pubmed publisher
	Wang M, Han W, Zhang M, Fang W, Zhai X, Guan S, et al. Long-term renal sympathetic denervation ameliorates renal fibrosis and delays the onset of hypertension in spontaneously hypertensive rats. Am J Transl Res. 2018;10:4042-4053 pubmed
	Nóbrega N, Araújo N, Reis D, Facine L, Miranda C, Mota G, et al. Hydrogen peroxide and nitric oxide induce anticontractile effect of perivascular adipose tissue via renin angiotensin system activation. Nitric Oxide. 2019;84:50-59 pubmed publisher
	Costa Fraga F, Goncalves G, Souza Neto F, Reis A, Capettini L, Santos R, et al. Age-related changes in vascular responses to angiotensin-(1-7) in female mice. J Renin Angiotensin Aldosterone Syst. 2018;19:1470320318789332 pubmed publisher
	Xiao H, Zhao L, Yang J, Tong N, An L, Liu Q, et al. Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest. Mol Med Rep. 2018;17:4221-4228 pubmed publisher
	Costa Besada M, Valenzuela R, Garrido Gil P, Villar Cheda B, Parga J, Lanciego J, et al. Paracrine and Intracrine Angiotensin 1-7/Mas Receptor Axis in the Substantia Nigra of Rodents, Monkeys, and Humans. Mol Neurobiol. 2018;55:5847-5867 pubmed publisher
	Burghi V, Fernández N, Gándola Y, Piazza V, Quiroga D, Guilhen Mario É, et al. Validation of commercial Mas receptor antibodies for utilization in Western Blotting, immunofluorescence and immunohistochemistry studies. PLoS ONE. 2017;12:e0183278 pubmed publisher
	Kranch Shorthouse R, Bauer A, Magness R, Lopez G, Segar J, Blohowiak S, et al. Ovine uterine space restriction causes dysregulation of the renin-angiotensin system in fetal kidneys. Biol Reprod. 2017;96:211-220 pubmed publisher
	Su Y, Bi J, Pulgar V, Chappell M, Rose J. Antenatal betamethasone attenuates the angiotensin-(1-7)-Mas receptor-nitric oxide axis in isolated proximal tubule cells. Am J Physiol Renal Physiol. 2017;312:F1056-F1062 pubmed publisher
	Ali Q, Dhande I, Samuel P, Hussain T. Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin II type 2 receptor/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed publisher
	Wilson B, Nautiyal M, Gwathmey T, Rose J, Chappell M. Evidence for a mitochondrial angiotensin-(1-7) system in the kidney. Am J Physiol Renal Physiol. 2016;310:F637-F645 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes: - 1-3. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013), (1:200).4-6. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).

image 2 :

Expression of Angiotensin-(1-7) Mas Receptor in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded sections using Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013), (1:100). Angiotensin-(1-7) Mas receptor (brown staining) is detected in proximal tubules (PT) and distal tubules (DT) in the renal cortex. Collecting ducts (CD) are less stained and both glomeruli (G) and blood vessels (A) are negative. Hematoxilin is used as the counterstain.

image 3 :

Western blot analysisof rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes: - 1-3. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013) (1:200).4-6. Anti-Angiotensin-(1-7) Mas Receptor Antibody preincubated with the control peptide antigen.

product information

CAT :

AAR-013

SKU :

AAR-013-CF_0.2 ml

Product Name :

Anti-Angiotensin-(1-7) Mas Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12526

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR013

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor

Product Page - Scientific background :

The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide Angiotensin-(1-7).1Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Proto-oncogene Mas, MAS1

Short Description :

A Rabbit Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor

Long Description :

Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse, and human samples.

Negative Control :

BLP-AR013

Positive Control :

Synonyms :

Proto-oncogene Mas, MAS1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MAS1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage