Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody | Alomone Labs AAR-013-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody

catalog :

AAR-013-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Madden J, Davis O, Boyle K, Iredale J, Browne T, Callister R, et al. Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice. Front Mol Neurosci. 2020;13:36 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1,2. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013-GP), (1:600).3,4. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).

product information

CAT :

AAR-013-GP

SKU :

AAR-013-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12526

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-AR013

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor

Product Page - Scientific background :

The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide angiotensin-(1-7).1 Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis. Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3 The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3 The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

Applications may also work in :

Supplier :

Alomone Labs

Target :

Proto-oncogene Mas, MAS1

Short Description :

A Guinea Pig Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013-GP, formerly AGP-099), raised in guinea pig can be used in western blot analysis. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-AR013

Positive Control :

Synonyms :

Proto-oncogene Mas, MAS1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MAS1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage