Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs AAR-012-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody

catalog :

AAR-012-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Dos Reis Costa D, Silveira A, Campos G, N xf3 brega N, de Ara xfa jo N, de Figueiredo Borges L, et al. High-Carbohydrate Diet Enhanced the Anticontractile Effect of Perivascular Adipose Tissue Through Activation of Renin-Angiotensin System. Front Physiol. 2020;11:628101 pubmed publisher
Le Gal L, Pellegrin M, Santoro T, Mazzolai L, Kurtz A, Meda P, et al. Connexin37-Dependent Mechanisms Selectively Contribute to Modulate Angiotensin II -Mediated Hypertension. J Am Heart Assoc. 2019;8:e010823 pubmed publisher

image

image 1 :

Expression of Angiotensin II receptor type-2 in rat brain - Immunohistochemical staining of rat frozen free floating brain sections using Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#AAR-012-AG), (1:20). AT2 receptor (green) was detected in neurons in the vicinity of the hypothalamic paraventricular nucleus (PVN). In some neurons (thick arrow), axonal processes with varicosities were observed (thin arrow). Nuclei were visualized with DAPI counterstain (blue).

image 2 :

Expression of Angiotensin II receptor type-2 inmouse 3T3-L1 cells - Cell surface detection ofAngiotensin II receptor type-2 in intact live mouse 3T3-L1 cells with Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488Antibody (#AAR-012-AG) (1:50) (green). Live view of the same field was superimposed to the fluorescent one.

product information

CAT :

AAR-012-AG

SKU :

AAR-012-AG_50 mcl

Product Name :

Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35351

Applications :

IC IF IHC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR012

Homology :

Mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminal domain

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor

Product Page - Scientific background :

Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

AT2 Receptor, AT2R, AGTR2, MRX88

Short Description :

A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won’t recognize human AT2R. Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#AAR-012-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging and immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

AT2 Receptor, AT2R, AGTR2, MRX88

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AGTR2

AB Specifictiy :

Will not recognize human AT2 Receptor.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-012), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:20-1:50

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage