Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody | Alomone Labs AAR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody

catalog :

AAR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 5a	Gallego Delgado J, Basu Roy U, Ty M, Alique M, Fernandez Arias C, Movila A, et al. Angiotensin receptors and ?-catenin regulate brain endothelial integrity in malaria. J Clin Invest. 2016;126:4016-4029 pubmed publisher
	Aparicio Prieto M, Rodr xed guez Gallego M, Valdivia Palac xed n A, Franco Iriarte Y, Herv xe1 s Barbara G, Echevarr xed a Orella E, et al. Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl. 2022;24:139-146 pubmed publisher
	Lee C, Park H, Lee B, Jeong S, Hyun S, Choi J, et al. Novel Therapeutic Effects of Pterosin B on Ang II-Induced Cardiomyocyte Hypertrophy. Molecules. 2020;25: pubmed publisher
	Raupp D, Fernandes R, Antunes K, Perin F, Rigatto K. Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis. Peptides. 2020;133:170384 pubmed publisher
	Qi R, Li T, Chen X. The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data. Ther Adv Endocrinol Metab. 2020;11:2042018820920560 pubmed publisher
	Singh D, Singh A, Singh D, Kesavan A, Arora S, Tiwary A, et al. Enhanced oral bioavailability and anti-diabetic activity of canagliflozin through a spray dried lipid based oral delivery: a novel paradigm. Daru. 2020;28:191-208 pubmed publisher
	Parekh R, Robidoux J, Sriramula S. Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons. Cell Mol Neurobiol. 2020;40:845-857 pubmed publisher
	Jeong J, Lee J, Lim J, Cho S, An S, Lee M, et al. Soluble RAGE attenuates AngII-induced endothelial hyperpermeability by disrupting HMGB1-mediated crosstalk between AT1R and RAGE. Exp Mol Med. 2019;51:1-15 pubmed publisher
	Cho S, Cho H, Woo K, Jeong J, Lim J, Park S, et al. Nelumbo nucifera Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy. Molecules. 2019;24: pubmed publisher
	Wang M, Han W, Zhang M, Fang W, Zhai X, Guan S, et al. Long-term renal sympathetic denervation ameliorates renal fibrosis and delays the onset of hypertension in spontaneously hypertensive rats. Am J Transl Res. 2018;10:4042-4053 pubmed
	Lei J, Zhang S, Wang P, Liao Y, Bian J, Yin X, et al. Long-term presence of angiotensin II type 1 receptor autoantibody reduces aldosterone production by triggering Ca2+ overload in H295R cells. Immunol Res. 2018;66:44-51 pubmed publisher
	da Silva J, Gabriel Costa D, Wang H, Ahmad S, Sun X, Varagic J, et al. Blunting of cardioprotective actions of estrogen in female rodent heart linked to altered expression of cardiac tissue chymase and ACE2. J Renin Angiotensin Aldosterone Syst. 2017;18:1470320317722270 pubmed publisher
	Tadevosyan A, Xiao J, Surinkaew S, Naud P, Merlen C, Harada M, et al. Intracellular Angiotensin-II Interacts With Nuclear Angiotensin Receptors in Cardiac Fibroblasts and Regulates RNA Synthesis, Cell Proliferation, and Collagen Secretion. J Am Heart Assoc. 2017;6: pubmed publisher
	Ismail B, dekemp R, Hadizad T, Mackasey K, Beanlands R, DaSilva J. Decreased renal AT1 receptor binding in rats after subtotal nephrectomy: PET study with [(18)F]FPyKYNE-losartan. EJNMMI Res. 2016;6:55 pubmed publisher

image

image 1 :

Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).

image 2 :

Expression of Angiotensin II Receptor Type-1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panels reveals partial co-localization.

product information

CAT :

AAR-011

SKU :

AAR-011-CF_0.2 ml

Product Name :

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30556

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR011

Homology :

Mouse, rat, rabbit, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor

Product Page - Scientific background :

Angiotensin II receptor type 1 (AT1 receptor, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (IP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by IP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

AT1 Receptor, AT1R, AGTR1

Short Description :

A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1

Long Description :

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.

Negative Control :

BLP-AR011

Positive Control :

Synonyms :

AT1 Receptor, AT1R, AGTR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AGTR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage