Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody | Alomone Labs AAR-011-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody

catalog :

AAR-011-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Raupp D, Fernandes R, Antunes K, Perin F, Rigatto K. Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis. Peptides. 2020;133:170384 pubmed publisher

image

image 1 :

Expression of AGTR1 in rat kidney - Immunohistochemical staining of rat kidney paraffin-embedded sections using Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO), (1:50), (red). Staining is specific to the innermost layer of the cortex. Intense staining is present in proximal tubes (P) but not in collecting ducts (C) in the cortical labyrinths (CL). No staining is present both in thin portions of the Loop of Henle or in the collecting ducts in the medullar rays (MR). Hoechst 33342 (blue) is used to visualize the nuclei.

image 2 :

Expression ofAGTR1 in rat kidney - Immunohistochemical staining of rat kidneyparaffin-embedded sections using Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550Antibody (#AAR-011-AO) (1:50) (red). Staining is specific to the innermost layer of the cortex. Intense staining is present in proximal tubes (P) but not in collecting ducts (C) in the cortical labyrinths (CL). No staining is present both in thin portions of the Loop of Henle or in the collecting ducts in the medullar rays (MR).Hoechst 33342 (blue) is used to visualize the nuclei.

image 3 :

Expression of Angiotensin II Receptor Type-1 in rat C6 glioma cells - Cell surface detection ofAngiotensin II Receptor Type-1 in live intact rat C6 glioma cells with Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550Antibody (#AAR-011-AO) (1:50) (left panel). Right panel shows live view of the same field.

product information

CAT :

AAR-011-AO

SKU :

AAR-011-AO_50 mcl

Product Name :

Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30556

Applications :

IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR011

Homology :

Mouse, rat, rabbit, dog - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor

Product Page - Scientific background :

Angiotensin II receptor type 1 (AT1, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

AT1 Receptor, AT1R, AGTR1

Short Description :

A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications, and will recognize AT1 receptor from rat, mouse and human samples. Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

AT1 Receptor, AT1R, AGTR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AGTR1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-011), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage