antibody

Anti-Adenosine A2A Receptor (extracellular)-PE Antibody

AAR-008-PE

polyclonal

domestic rabbit

PE

NA

mouse, rat

AAR-008-PE_15 mcl

Anti-Adenosine A2A Receptor (extracellular)-PE Antibody

Antibodies

Antibodies

Polyclonal

Q60613

FC

Rat Mouse

Rabbit

BLP-AR008

Mouse - 13 out of 14 amino acid residues identical; rat - 12 out of 14 amino acid residues identical It will not recognize A2A Adenosine Receptor from human samples

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

2nd extracellular loop

R-Phycoerythrin (R-PE).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A2A receptor

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins. The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

FC

Alomone Labs

ADORA2A, A2AAR, Adenosine A2aR

A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)

Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize A2aR from mouse and rat samples. The antibody will not recognize the receptor from human samples. Anti-Adenosine A2A Receptor (extracellular)-PE Antibody (#AAR-008-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-PE

NA

ADORA2A, A2AAR, Adenosine A2aR

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADORA2A

It will not recognize A2A Adenosine Receptor from human samples

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #AAR-008), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information