Anti-Human Adenosine A2A Receptor (extracellular) Antibody | Alomone Labs AAR-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human Adenosine A2A Receptor (extracellular) Antibody

catalog :

AAR-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Newton H, Chimote A, Arnold M, Wise Draper T, Conforti L. Targeted knockdown of the adenosine A2A receptor by lipid NPs rescues the chemotaxis of head and neck cancer memory T cells. Mol Ther Methods Clin Dev. 2021;21:133-143 pubmed publisher
Han B, Lin S, Espinosa K, Thorne P, Vlajkovic S. Inhibition of the Adenosine A2A Receptor Mitigates Excitotoxic Injury in Organotypic Tissue Cultures of the Rat Cochlea. Cells. 2019;8: pubmed publisher
Grillo S, McDevitt D, Voas M, Khan A, Grillo M, Stella S. Adenosine receptor expression in the adult zebrafish retina. Purinergic Signal. 2019;15:327-342 pubmed publisher

image

image 1 :

Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4): - 1,2. Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007), (1:200).3,4. Anti-Human Adenosine A2A Receptor (extracellular) Antibody, preincubated with Human Adenosine A2A Receptor (extracellular) Blocking Peptide (#BLP-AR007).

product information

CAT :

AAR-007

SKU :

AAR-007-CF_0.2 ml

Product Name :

Anti-Human Adenosine A2A Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P29274

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-AR007

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CGQPKEGKNHSQG, corresponding to amino acid residues 146 - 158 of human adenosine A2A receptor

Product Page - Scientific background :

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Adenosine receptor A2A, A2AR, ADORA2A

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A2A Receptor

Long Description :

Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize A2AR from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

Negative Control :

BLP-AR007

Positive Control :

Synonyms :

Adenosine receptor A2A, A2AR, ADORA2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA2A

AB Specifictiy :

Will not recognize rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage