Anti-Adenosine A1 Receptor Antibody | Alomone Labs AAR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenosine A1 Receptor Antibody

catalog :

AAR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Zhao Z, Shang X, Chen Y, Zheng Y, Huang W, Jiang H, et al. Bacteria elevate extracellular adenosine to exploit host signaling for blood-brain barrier disruption. Virulence. 2020;11:980-994 pubmed publisher
Hong S, Bullert A, Baker M, Choi D. Astrocytic equilibrative nucleoside transporter type 1 upregulations in the dorsomedial and dorsolateral striatum distinctly coordinate goal-directed and habitual ethanol-seeking behaviours in mice. Eur J Neurosci. 2020;52:3110-3123 pubmed publisher
Lin S, Thorne P, Housley G, Vlajkovic S. Purinergic Signaling and Aminoglycoside Ototoxicity: The Opposing Roles of P1 (Adenosine) and P2 (ATP) Receptors on Cochlear Hair Cell Survival. Front Cell Neurosci. 2019;13:207 pubmed publisher
Yan A, Joachims M, Thompson L, Miller A, Canoll P, Bynoe M. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. J Neurosci. 2019;39:4387-4402 pubmed publisher
Hackett T. Adenosine A1 Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain. Anat Rec (Hoboken). 2018;301:1882-1905 pubmed publisher
Sureechatchaiyan P, Hamacher A, Brockmann N, Stork B, Kassack M. Adenosine enhances cisplatin sensitivity in human ovarian cancer cells. Purinergic Signal. 2018;14:395-408 pubmed publisher
Jiang S, Lin Y, Hsieh C. Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABAA Receptors in Rat Spinal Cord. Evid Based Complement Alternat Med. 2018;2018:8041820 pubmed publisher
Wu L, Meng J, Shen Q, Zhang Y, Pan S, Chen Z, et al. Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice. Nat Commun. 2017;8:15904 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1, 3) and rat kidney (lanes 2, 4) lysates: - 1,2. Anti-Adenosine A1 Receptor Antibody (#AAR-006), (1:200).3,4. Anti-Adenosine A1 Receptor Antibody, preincubated with Adenosine A1 Receptor Blocking Peptide (#BLP-AR006).

image 2 :

Expression of Adenosine A1 Receptor in rat cortex - Immunohistochemical staining of rat cortex frozen section using Anti-Adenosine A1 Receptor Antibody (#AAR-006). A. A1AR (green) appears in neurons (triangles) and in astrocytes (arrows). B. Parvalbumin staining (red) appears in cortical interneurons. C. Confocal merge of images demonstrates the existence of A1AR in a subset of cortical interneurons and astrocytes.

image 3 :

Expression of AdenosineA1Receptor in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.A. Staining ofDRG cells with Anti-AdenosineA1Receptor Antibody (#AAR-006) (1:100) followed by goat anti-rabbit AlexaFluor-555 secondary antibody.B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. Merged images of A and B.F. Merged images of D and E.Magnification:A-C: x20D-F: x100

product information

CAT :

AAR-006

SKU :

AAR-006-CF_0.2 ml

Product Name :

Anti-Adenosine A1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30542

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR006

Homology :

Mouse, rat, bovine, canis and pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR

Product Page - Scientific background :

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

ADORA1, A1AR, Adenosine A1R

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A1 Receptor

Long Description :

Alomone Labs is pleased to introduce a new antibody directed against human adenosine A1 receptor. Anti-Adenosine A1 Receptor Antibody (#AAR-006) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize adenosine A1R from rat, mouse and human samples.

Negative Control :

BLP-AR006

Positive Control :

Synonyms :

ADORA1, A1AR, Adenosine A1R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage