Anti-Adenosine A3 Receptor Antibody | Alomone Labs AAR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenosine A3 Receptor Antibody

catalog :

AAR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Coppi E, Cherchi F, Fusco I, Failli P, Vona A, Dettori I, et al. Adenosine A3 receptor activation inhibits pronociceptive N-type Ca2+ currents and cell excitability in dorsal root ganglion neurons. Pain. 2019;160:1103-1118 pubmed publisher
Yan A, Joachims M, Thompson L, Miller A, Canoll P, Bynoe M. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. J Neurosci. 2019;39:4387-4402 pubmed publisher
Sureechatchaiyan P, Hamacher A, Brockmann N, Stork B, Kassack M. Adenosine enhances cisplatin sensitivity in human ovarian cancer cells. Purinergic Signal. 2018;14:395-408 pubmed publisher

image

image 1 :

Western blot analysis of Malme-3M cell lysate: - 1. Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:200).2. Anti-Adenosine A3 Receptor Antibody, preincubated with Adenosine A3 Receptor Blocking Peptide (#BLP-AR004).

image 2 :

Expression of Adenosine A3 Receptor in rat lung - Immunohistochemical staining of paraffin-embedded rat lung sections using Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (arrows) and pneumonocytes in the alveolar wall (a). Hematoxilin is used as the counterstain.

image 3 :

Peptide (C)KETGAFYGREFKTAK corresponding to amino acid residues 216-230 of human A3AR (Accession P0DMS8). 3rd intracellular loop.

product information

CAT :

AAR-004

SKU :

AAR-004-CF_0.2 ml

Product Name :

Anti-Adenosine A3 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P0DMS8

Applications :

IC IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR004

Homology :

Rat, mouse - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR

Product Page - Scientific background :

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

ADORA3, A3AR, Adenosine A3R

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A3 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human adenosine A3 receptor (A3AR). Anti-Adenosine A3 Receptor Antibody (#AAR-004) can be used in western blot analysis, as well as immunocytochemical and immunohistocemical applications, and has been designed to recognize A3AR from human, rat, and mouse samples.

Negative Control :

BLP-AR004

Positive Control :

Synonyms :

ADORA3, A3AR, Adenosine A3R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage