Anti-Adenosine A2B Receptor (extracellular) Antibody | Alomone Labs AAR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenosine A2B Receptor (extracellular) Antibody

catalog :

AAR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 12

Reference
Campos Contreras A, Gonz xe1 lez Gallardo A, D xed az Mu xf1 oz M, V xe1 zquez Cuevas F. Adenosine Receptor A2B Negatively Regulates Cell Migration in Ovarian Carcinoma Cells. Int J Mol Sci. 2022;23: pubmed publisher
Tang J, Zou Y, Li L, Lu F, Xu H, Ren P, et al. BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b Receptor. Front Pharmacol. 2021;12:619800 pubmed publisher
Mishra V, Kumar N, Raza M, Sehrawat S. Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme. Oncotarget. 2020;11:4754-4769 pubmed publisher
Yan A, Joachims M, Thompson L, Miller A, Canoll P, Bynoe M. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. J Neurosci. 2019;39:4387-4402 pubmed publisher
Gardner J, Jackaman C, Mamotte C, Nelson D. The Regulatory Status Adopted by Lymph Node Dendritic Cells and T Cells During Healthy Aging Is Maintained During Cancer and May Contribute to Reduced Responses to Immunotherapy. Front Med (Lausanne). 2018;5:337 pubmed publisher
Sureechatchaiyan P, Hamacher A, Brockmann N, Stork B, Kassack M. Adenosine enhances cisplatin sensitivity in human ovarian cancer cells. Purinergic Signal. 2018;14:395-408 pubmed publisher
Dorotea D, Cho A, Lee G, Kwon G, Lee J, Sahu P, et al. Orally active, species-independent novel A3 adenosine receptor antagonist protects against kidney injury in db/db mice. Exp Mol Med. 2018;50:38 pubmed publisher
Lavoie E, Fausther M, Goree J, Dranoff J. The Cholangiocyte Adenosine-IL-6 Axis Regulates Survival During Biliary Cirrhosis. Gene Expr. 2017;17:327-340 pubmed publisher
Densmore J, Schaid T, Jeziorczak P, Medhora M, Audi S, Nayak S, et al. Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia. Exp Lung Res. 2017;43:38-48 pubmed publisher
Andrés R, Terencio M, Arasa J, Paya M, Valcuende Cavero F, Navalón P, et al. Adenosine A2A and A2B Receptors Differentially Modulate Keratinocyte Proliferation: Possible Deregulation in Psoriatic Epidermis. J Invest Dermatol. 2017;137:123-131 pubmed publisher
Benito MuÃ±oz M, Matute C, Cavaliere F. Adenosine A1 receptor inhibits postnatal neurogenesis and sustains astrogliogenesis from the subventricular zone. Glia. 2016;64:1465-78 pubmed publisher
Hayashi M, Inagaki A, Novak I, Matsuda H. The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells. Pflugers Arch. 2016;468:1171-81 pubmed publisher

image

image 1 :

Western blot analysis of HL-60 (lanes 1 and 2) cell line, rat brain (lanes 3 and 4) and mouse brain (lanes 5 and 6) lysates: - 1,3,5. Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003), (1:200).2,4,6. Anti-Adenosine A2B Receptor (extracellular) Antibody, preincubated with Adenosine A2B Receptor (extracellular) Blocking Peptide (#BLP-AR003).

image 2 :

Expression of Adenosine A2B Receptor in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003), (1:50). Staining is present in the respiratory epithelium of the bronchiole (Br) as well as in the pneumonocytes of the alveolar wall (alveoli, (A). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.

image 3 :

Expression of AdenosineA2BReceptorin rat PC12 cells - Cell surface detection ofAdenosineA2BReceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-AdenosineA2BReceptor (extracellular)Antibody (#AAR-003)(1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merge of A and live view of the cells.

product information

CAT :

AAR-003

SKU :

AAR-003-CF_0.2 ml

Product Name :

Anti-Adenosine A2B Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P29275

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR003

Homology :

Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S)

Product Page - Scientific background :

Extracellular adenosine is a ubiquitous signaling molecule that modulates a wide array of biological processes. There are currently four known sub-types of adenosine receptors: A1, A2A, A2B and A3.The adenosine A2B receptor is a typical class A, G protein-coupled receptor with seven transmembrane segments, an extracellular N-terminus and an intracellular C-terminus. Adenosine A2BR causes an increase in intracellular cAMP levels by coupling mainly to Gs proteins, resulting in the activation of adenylyl cyclase1 and subsequent activation of PKA and other cAMP effectors such as Epac. In addition, the A2BR couples to Gq and activates the PLC pathway that mediates several crucial functions of A2BR. A2BR also couples to the MAPK and arachidonic acid signaling pathways and regulates membrane ion channels probably through G-protein βγ subunits.Multiple molecules that bind to A2BR have been identified in an effort to understand the receptor's function and regulation. NFκB1/p105 is a member of the NFκB family of proteins that perform regulatory functions in diverse biological processes such as inflammation and cell survival and differentiation, as well as in various diseases. Interestingly, Although A2BR activation by adenosine produces pro-inflammatory effects the receptor also binds to specific sites on p105, prevents the poly-ubiquitination and degradation of the p105 protein and inhibits NFκB activation thus reducing inflammation.A2BR is highly expressed in various types of tumor cells or tissues and promotes tumor-cell proliferation. A2BR was found to be overexpressed in colorectal carcinoma cells and tissues, and inhibition of A2BR blocks the proliferation of colon cancer cells. A2BR has been implicated in the pathophysiology of vascular diseases, renal disease and diabetes2.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

ADORA2b, A2BAR, Adenosine A2bR

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A2B Receptor

Long Description :

Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003) is a highly specific antibody directed against an extracellular epitope of human adenosine A2B receptor (A2BAR). The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize A2BAR from human, rat and mouse samples.

Negative Control :

BLP-AR003

Positive Control :

Synonyms :

ADORA2b, A2BAR, Adenosine A2bR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA2B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage