antibody

Anti-Adenosine A2B Receptor (extracellular)-APC Antibody

AAR-003-APC

polyclonal

domestic rabbit

APC

NA

human, mouse, rat

AAR-003-APC_50 mcl

Anti-Adenosine A2B Receptor (extracellular)-APC Antibody

Antibodies

Antibodies

Polyclonal

P29275

FC LCI

Human Rat Mouse

Rabbit

BLP-AR003

Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

2nd extracellular loop

Allophycocyanin (APC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S)

Extracellular adenosine is a ubiquitous signaling molecule that modulates a wide array of biological processes. There are currently four known sub-types of adenosine receptors: A1, A2A, A2B and A3.The adenosine A2B receptor is a typical class A, G protein-coupled receptor with seven transmembrane segments, an extracellular N-terminus and an intracellular C-terminus. Adenosine A2BR causes an increase in intracellular cAMP levels by coupling mainly to Gs proteins, resulting in the activation of adenylyl cyclase1 and subsequent activation of PKA and other cAMP effectors such as Epac. In addition, the A2BR couples to Gq and activates the PLC pathway that mediates several crucial functions of A2BR. A2BR also couples to the MAPK and arachidonic acid signaling pathways and regulates membrane ion channels probably through G-protein βγ subunits.Multiple molecules that bind to A2BR have been identified in an effort to understand the receptor's function and regulation. NFκB1/p105 is a member of the NFκB family of proteins that perform regulatory functions in diverse biological processes such as inflammation and cell survival and differentiation, as well as in various diseases. Interestingly, Although A2BR activation by adenosine produces pro-inflammatory effects the receptor also binds to specific sites on p105, prevents the poly-ubiquitination and degradation of the p105 protein and inhibits NFκB activation thus reducing inflammation.A2BR is highly expressed in various types of tumor cells or tissues and promotes tumor-cell proliferation. A2BR was found to be overexpressed in colorectal carcinoma cells and tissues, and inhibition of A2BR blocks the proliferation of colon cancer cells. A2BR has been implicated in the pathophysiology of vascular diseases, renal disease and diabetes2.

FC LCI

Alomone Labs

ADORA2b, A2BAR, Adenosine A2b Receptor

A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Adenosine A2b Receptor from human, mouse, and rat samples. Anti-Adenosine A2B Receptor (extracellular)-APC Antibody (#AAR-003-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-APC

NA

ADORA2b, A2BAR, Adenosine A2b Receptor

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADORA2B

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #AAR-003), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information