Anti-Adenosine A2A Receptor Antibody | Alomone Labs AAR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenosine A2A Receptor Antibody

catalog :

AAR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Skop xe1 l A, K xe9 ki T, T xf3 th P, Cs xf3 ka B, Koscs xf3 B, N xe9 meth Z, et al. Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling. J Biol Chem. 2022;298:101888 pubmed publisher
Jagannath A, Varga N, Dallmann R, Rando G, Gosselin P, Ebrahimjee F, et al. Adenosine integrates light and sleep signalling for the regulation of circadian timing in mice. Nat Commun. 2021;12:2113 pubmed publisher
Han B, Lin S, Espinosa K, Thorne P, Vlajkovic S. Inhibition of the Adenosine A2A Receptor Mitigates Excitotoxic Injury in Organotypic Tissue Cultures of the Rat Cochlea. Cells. 2019;8: pubmed publisher
Yan A, Joachims M, Thompson L, Miller A, Canoll P, Bynoe M. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling. J Neurosci. 2019;39:4387-4402 pubmed publisher
Sureechatchaiyan P, Hamacher A, Brockmann N, Stork B, Kassack M. Adenosine enhances cisplatin sensitivity in human ovarian cancer cells. Purinergic Signal. 2018;14:395-408 pubmed publisher
Densmore J, Schaid T, Jeziorczak P, Medhora M, Audi S, Nayak S, et al. Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia. Exp Lung Res. 2017;43:38-48 pubmed publisher
Andrés R, Terencio M, Arasa J, Paya M, Valcuende Cavero F, Navalón P, et al. Adenosine A2A and A2B Receptors Differentially Modulate Keratinocyte Proliferation: Possible Deregulation in Psoriatic Epidermis. J Invest Dermatol. 2017;137:123-131 pubmed publisher
Benito MuÃ±oz M, Matute C, Cavaliere F. Adenosine A1 receptor inhibits postnatal neurogenesis and sustains astrogliogenesis from the subventricular zone. Glia. 2016;64:1465-78 pubmed publisher
Ingwersen J, Wingerath B, Graf J, Lepka K, Hofrichter M, SchrÃ¶ter F, et al. Dual roles of the adenosine A2a receptor in autoimmune neuroinflammation. J Neuroinflammation. 2016;13:48 pubmed publisher

image

image 1 :

Western blot analysis of rat aortic endothelial cells (lanes 1, 3), rat brain (lanes 2, 4) and Jurkat (lanes 5, 6) lysates: - 1,2,5. Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:200).3,4,6. Anti-Adenosine A2A Receptor Antibody, preincubated with Adenosine A2A Receptor Blocking Peptide (#BLP-AR002).

image 2 :

Expression of Adenosine A2A Receptor in mouse diagonal band - Immunohistochemical staining of mouse diagonal band using Anti-Adenosine A2A Receptor Antibody (#AAR-002). A2aR (green) appears in the broca of individual neurons (arrows) and in neuropil (asterisk). DAPI is used as the counterstain.

image 3 :

Expression of AdenosineA2AReceptorin rat lung - Immunohistochemical staining of paraffin emedded rat lung sections using Anti-AdenosineA2AReceptorAntibody (#AAR-002) (1:50). A2aR is expressed in the respiratory epithelium of the bronchioli (Br). Note that smooth muscle and endothelium in blood vessels are negative. Hematoxilin is used as the counterstain.

product information

CAT :

AAR-002

SKU :

AAR-002-CF_0.2 ml

Product Name :

Anti-Adenosine A2A Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q60613

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR002

Homology :

Rat, human, and canis - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor

Product Page - Scientific background :

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS.5,6A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues.8

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

ADORA2A, A2AAR, Adenosine A2aR

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A2A Receptor

Long Description :

Anti-Adenosine A2A Receptor Antibody (#AAR-002) is a highly specific antibody directed against an epitope of mouse protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize A2aR from human, rat, and mouse samples.

Negative Control :

BLP-AR002

Positive Control :

Synonyms :

ADORA2A, A2AAR, Adenosine A2aR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage