Anti-ACE2 (extracellular) Antibody | Alomone Labs AAC-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ACE2 (extracellular) Antibody

catalog :

AAC-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat small intestine lysate (lanes 1 and 4), mouse lung lysate (lanes 2 and 5 ) and mouse kidney lysate (lanes 3 and 6): - 1-3. Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200). 4-6. Anti-ACE2 (extracellular) Antibody, preincubated with ACE2 (extracellular) Blocking Peptide (BLP-AC012).

image 2 :

Expression of ACE2 in mouse thalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. ACE2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with ACE2 (extracellular) Blocking Peptide (BLP-AC012), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AAC-012

SKU :

AAC-012-CF_0.2 ml

Product Name :

Anti-ACE2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BYF1

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AC012

Homology :

Rat - 14 out of 17 amino acid residues identical, mouse - 13 out of 17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EDYGDYWRGDYEVNGVD, corresponding to amino acid residues 197 - 213 of human ACE2

Product Page - Scientific background :

The renin-angiotensin system is a central mechanism for blood pressure regulation through a diverse system of hormones and receptors. Angiotensin-converting enzyme (ACE) is an ectoenzyme that plays a role in the generation of Ang II by catalyzing the extracellular conversion of the decapeptide Ang I. Moreover, ACE degrades active bradykinin (BK), which play an important role in the control of blood pressure. ACE2 is an ACE homolog that was discovered in 2000 when two independent research groups cloned homologous ACE that could convert Ang I to Ang (1-9). Sequence analysis suggests that ACE and ACE2 exhibit 42% amino acid homology and ACE2 has evolved through gene duplication1.ACE2 consist of N-terminal peptidase domain with seven potential N-linked glycosylation sites that bears the characteristic HEXXH motif of M2 metalloproteases. It is mainly expressed in the small intestine, testis, kidneys, heart, thyroid and adipose tissue and in the lungs. ACE 2 has a protective role in the cardiovascular system and in alveolar epithelial cells. In the lungs ACE2 has numerous physiological functions, most of which are protective against lung injury2. ACE 2 was identified as the receptor for highly pathogenic SARS (severe acute respiratory syndrome) coronavirus including the SARS-CoV-2 that cause the coronavirus disease 2019 (COVID-19) global pandemic. SARS virus binding downregulates the cellular expression of ACE2, and the binding induces clathrin-dependent internalization of virus/receptor (SARS/ACE2) complex. Not only has ACE2 facilitated the invasion of SARS virus for rapid replication, but also ACE2 is depleted from the cell membrane and therefore the damaging effects of Ang II are enhanced, resulting in acute deterioration of lung tissues3.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

Short Description :

A Rabbit Polyclonal Antibody to ACE2 (extracellular)

Long Description :

Anti-ACE2 (extracellular) Antibody (#AAC-012) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ACE2 from rat, mouse and human samples.

Negative Control :

BLP-AC012

Positive Control :

Synonyms :

Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ACE2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage