Anti-ACE2 (extracellular)-FITC Antibody | Alomone Labs AAC-012-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ACE2 (extracellular)-FITC Antibody

catalog :

AAC-012-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of ACE2 by direct flow cytometry in live intact mouse M1 myeloid leukemia cell line:

image 2 :

Cell surface detection of ACE2 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:

product information

CAT :

AAC-012-F

SKU :

AAC-012-F_50 mcl

Product Name :

Anti-ACE2 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BYF1

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AC012

Homology :

Rat - 14 out of 17 amino acid residues identical, mouse - 13 out of 17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-term.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EDYGDYWRGDYEVNGVD, corresponding to amino acid residues 197 - 213 of human ACE2

Product Page - Scientific background :

The renin-angiotensin system is a central mechanism for blood pressure regulation through a diverse system of hormones and receptors. Angiotensin-converting enzyme (ACE) is an ectoenzyme that plays a role in the generation of Ang II by catalyzing the extracellular conversion of the decapeptide Ang I. Moreover, ACE degrades active bradykinin (BK), which play an important role in the control of blood pressure. ACE2 is an ACE homolog that was discovered in 2000 when two independent research groups cloned homologous ACE that could convert Ang I to Ang (1-9). Sequence analysis suggests that ACE and ACE2 exhibit 42% amino acid homology and ACE2 has evolved through gene duplication1.ACE2 consist of N-terminal peptidase domain with seven potential N-linked glycosylation sites that bears the characteristic HEXXH motif of M2 metalloproteases. It is mainly expressed in the small intestine, testis, kidneys, heart, thyroid and adipose tissue and in the lungs. ACE 2 has a protective role in the cardiovascular system and in alveolar epithelial cells. In the lungs ACE2 has numerous physiological functions, most of which are protective against lung injury2. ACE 2 was identified as the receptor for highly pathogenic SARS (severe acute respiratory syndrome) coronavirus including the SARS-CoV-2 that cause the coronavirus disease 2019 (COVID-19) global pandemic. SARS virus binding downregulates the cellular expression of ACE2, and the binding induces clathrin-dependent internalization of virus/receptor (SARS/ACE2) complex. Not only has ACE2 facilitated the invasion of SARS virus for rapid replication, but also ACE2 is depleted from the cell membrane and therefore the damaging effects of Ang II are enhanced, resulting in acute deterioration of lung tissues3.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

Short Description :

A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-ACE2 (extracellular) Antibody (#AAC-012) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize ACE2 from mouse, rat and human samples. Anti-ACE2 (extracellular)-FITC Antibody (#AAC-012-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ACE2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAC-012), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage