Anti-LRRC8A (extracellular) Antibody | Alomone Labs AAC-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LRRC8A (extracellular) Antibody

catalog :

AAC-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Kandashvili M, Gamkrelidze G, Tsverava L, Lordkipanidze T, Lepsveridze E, Lagani V, et al. Myo-Inositol Limits Kainic Acid-Induced Epileptogenesis in Rats. Int J Mol Sci. 2022;23: pubmed publisher
Egorov Y, Lang D, Tyan L, Turner D, Lim E, Piro Z, et al. Caveolae-Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis. J Am Heart Assoc. 2019;8:e012748 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate: - 1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).

image 2 :

Expression of LRRC8A in mouse cerebellum and olfactory bulb - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. LRRC8A staining in mouse cerebellum (green) shows intensely positive cells (arrows) with Bergmann glia morphology and localization. B. Staining in mouse olfactory bulb shows intensely positive cells (arrows) with glial morphology in some glomeruli (G). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AAC-001

SKU :

AAC-001-CF_0.2 ml

Product Name :

Anti-LRRC8A (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8IWT6

Applications :

IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AC001

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DTGPTGIKYDLDRH, corresponding to amino acid residues 91-104 of human LRRC8A

Product Page - Scientific background :

Leucine-rich repeat-containing 8 A (LRRC8A, SWELL1) is an essential component of the volume-regulated anion channel (VRAC) that is activated by cell swelling and ionic strength. In response to swelling, mammalian cells release chloride and organic osmolytes through VRAC channels. LRRC8A serves as a sensor of ionic strength gradient in the absence of osmotic gradient. In addition, LRRC8A plays a role in the uptake of chemotherapeutic agents1-3.LRRC8A protein structure contains 4 transmembrane segments and about 17 leucine-rich repeats (LRRs) at the cytoplasmic C-terminus3. VRAC channels are composed of the LRRC8A subunit and at least one of LRRC8B-E subunits. Deletion of LRRC8A greatly decreases the hypo-osmolarity-induced current1,3.LRRC8A mRNA and protein are expressed in several neuronal tissues including brain stem ganglia, nodose ganglia and dorsal root ganglia. LRRC8A is also detected in the heart, aorta, and small intestine1.

Applications may also work in :

IF IFC LCI WB

Supplier :

Alomone Labs

Target :

Leucine-rich repeat-containing protein 8A, Volume-regulated anion channel subunit LRRC8A, LRRC8, Swelling protein 1, SWELL1

Short Description :

A Rabbit Polyclonal Antibody to LRRC8A

Long Description :

Anti-LRRC8A (extracellular) Antibody (#AAC-001) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SWELL1 from rat, mouse, and human samples.

Negative Control :

BLP-AC001

Positive Control :

Synonyms :

Leucine-rich repeat-containing protein 8A, Volume-regulated anion channel subunit LRRC8A, LRRC8, Swelling protein 1, SWELL1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRC8A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage