A23187 | Alomone Labs A-600 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

A23187

catalog :

A-600

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Berberián M, Pocognoni C, Mayorga L. A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells. Int J Nanomedicine. 2018;13:8075-8086 pubmed publisher
Uchida M, Ito F, Tsuchiya T, Shoji Y, Kurosawa T. Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation. Exp Anim. 2015;64:333-41 pubmed publisher
Sosa C, Pavarotti M, Zanetti M, Zoppino F, De Blas G, Mayorga L. Kinetics of human sperm acrosomal exocytosis. Mol Hum Reprod. 2015;21:244-54 pubmed publisher
Páramo B, Montiel T, Hernández Espinosa D, Rivera Martínez M, Moran J, Massieu L. Calpain activation induced by glucose deprivation is mediated by oxidative stress and contributes to neuronal damage. Int J Biochem Cell Biol. 2013;45:2596-604 pubmed publisher
Rodriguez Peña M, Castillo Bennett J, Soler O, Mayorga L, Michaut M. MARCKS protein is phosphorylated and regulates calcium mobilization during human acrosomal exocytosis. PLoS ONE. 2013;8:e64551 pubmed publisher
Suhaiman L, De Blas G, Obeid L, Darszon A, Mayorga L, Belmonte S. Sphingosine 1-phosphate and sphingosine kinase are involved in a novel signaling pathway leading to acrosomal exocytosis. J Biol Chem. 2010;285:16302-14 pubmed publisher
A F, C J, H J A. Properties of the SR Ca-ATPase in an Open Microsomal Membrane Preparation. Open Biochem J. 2008;2:91-9 pubmed publisher

product information

CAT :

A-600

SKU :

A-600_1 mg

Product Name :

A23187

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

A23187 is a mobile-carrier Ca2+ ionophore (allows Ca2+ ions to cross cell membranes) originally isolated as an antibiotic from Streptomyces chartreusensis. It is used to increase intracellular Ca2+ levels and for in vitro studies related to intracellular Ca2+-mediated events. A23187 can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.1,2Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+-triggered cell apoptosis.3,4 This property was exploited to clarify apoptotic events in different cell lines such as mouse lymphoma cell line (S49),5,6 histiocytic lymphoma U937,7 human myeloid leukemia cells (HL-60),8 cultured striatal neurons,9 renal tubular cells,10 and C6 glioma cells.11

Supplier :

Alomone Labs

Long Description :

A23187 (#A-600) is a highly pure, natural, and biologically active compound.

Short Description :

Widely Used Ca2+ Ionophore

MW :

523.6

Synonyms :

Calcimycin, Calcium ionophore A23187

Source :

Natural

Molecular formula :

C29H37N3O6.

Effective Concentration :

1-10 µM.

Activity :

A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors1,2. Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+ triggered cell apoptosis3,4.

Reconstitution and Solubility :

DMSO, ethanol or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO, ethanol or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

52665-69-7

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11- trimethy l-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7- dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid.

more info or order :

Alomone Labs product webpage