Anisomycin | Alomone Labs A-520 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Anisomycin

catalog :

A-520

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Takino J, Sato T, Nagamine K, Hori T. The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells. Sci Rep. 2019;9:16717 pubmed publisher
Villers A, Godaux E, Ris L. Long-lasting LTP requires neither repeated trains for its induction nor protein synthesis for its development. PLoS ONE. 2012;7:e40823 pubmed publisher

image

image 1 :

Alomone Labs Ergtoxin-1 inhibits the current of KV11.1 (hErg) channels expressed inXenopusoocytes. - A. Representative time course ofKV11.1 current inhibition by 50 nM Ergtoxin-1(#STE-450) as indicated (green). B. Superimposed traces of KV11.1 current after application of control solution (black) and of 50 nM Ergtoxin-1 (green) taken from the recording in A. Membrane potential was held at -100 mV. Current was elicited every 10 sec by a 200 ms voltage step to +40 mV followed by a 200 ms step to -40 mV (as shown below).

product information

CAT :

A-520

SKU :

A-520_10 mg

Product Name :

Anisomycin

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Anisomycin is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis by binding to 60S ribosomal subunits and blocking peptide bond formation, thereby preventing elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2/p38MAPK), kinase subtypes and p46/54JNK.6-13Synergizes with growth factors and phorbol esters, anisomycin superinduce c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15

Supplier :

Alomone Labs

Long Description :

Anisomycin (#A-520) is a highly pure, natural, and biologically active compound.

Short Description :

Protein Synthesis Inhibitor

MW :

265.3

Synonyms :

Flagecidin

Source :

Natural

Molecular formula :

C14H19NO4.

Effective Concentration :

0.1-10 µM.

Activity :

Anisomycin is an antibiotic which inhibits protein synthesis1. It also activates stress-activated protein kinases (SAPK) independent of its ability to block translation.

Reconstitution and Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

22862-76-6

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate.

more info or order :

Alomone Labs product webpage