A-794278 | Alomone Labs A-435 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

A-794278

catalog :

A-435

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4) rat brain (lanes 2 and 5) and human brain neuroblastoma cells (SH-SY5Y) (lanes 3 and 6): - 1-3. Guinea pigAnti-CaV1.3(CACNA1D)Antibody (#AGP-061) (1:200).4-6. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody preincubated with the negative control antigen.Following a broad screen of secondary antibodies the following were used for this application:Western blot analysis:#106-035-006 (Jackson ImmunoResearch)#A7289 (Sigma)

product information

CAT :

A-435

SKU :

A-435_10 mg

Product Name :

A-794278

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

A-794278 is a potent, non-competitive antagonist of mGluR1. It is a triazafluorenone derivative that also antagonizes mGluR5 receptors. It demonstrates IC50 values of 4.1 nM and 147 nM for mGluR1 and mGluR5, respectively1. A-794278 binds to a putative allosteric recognition site located within the seven-transmembrane domain of the receptor1,2.Glutamate is the most abundant excitatory neurotransmitter in the central nervous system. It modulates the activity of many types of synapses by activating in part metabotropic glutamate receptors (mGluRs). mGluRs consist of three groups: I, II, and III with a total of eight subtypes, mGluR1 to mGluR8. mGluR1 plays an important role in the central sensitization of pain and in a variety of functions with potential implications in neurological and psychiatric disorders1,2.

Supplier :

Alomone Labs

Target :

mGluR1, mGluR5

Long Description :

A-794278 (#A-435) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Antagonist of mGluR1 and mGluR5

MW :

342.46

Source :

Synthetic

Molecular formula :

C18H22N4OS.

Effective Concentration :

1-100 nM.

Activity :

A-794278 is a potent and selective noncompetitive mGluR1 antagonist, acting on mGluR1 and mGluR5 with IC50 values of 4.1 nM and 147 nM, respectively1. In vivo it shows analgesic properties, significantly attenuating spontaneous post-operative pain behavior in rats (ED50 = 50 μMol/kg)2.

Reconstitution and Solubility :

Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

-20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

869802-57-3

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

3-cycloheptyl-9-(dimethylamino)pyrido[1,2]thieno[3,4-d]pyrimidin-4-one.

more info or order :

Alomone Labs product webpage