chemical

AP-18

A-250

A-250_10 mg

AP-18

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

The transient receptor potential (TRP) channel is one of the largest families of cation channels1. TRPA1 is expressed in sensory and nociceptive neurons and has a significant role in inflammatory responses. Bradykinin and histamine receptors induce TRPA1 activity. A study conducted found that TRPA1 deficient mice did not develop an elevated mechanical and thermal pain response following bradykinin injection, suggesting that TRPA1 has a vital role in bradykinin signaling pathways2.TRPA1 also plays a role in acute and chronic respiratory disease pathophysiology. Prolonged exposure to TRPA1 agonists such as chlorine and aldehydes can cause reactive airways dysfunction syndrome characterized by symptoms such as wheezing, coughing and dyspnea. In light of this, TRPA1 may be a target for airway anti-inflammatory research3.AP-18 is a reversible TRPA1 blocker with an IC50 value of 3.1 and 4.5 μM for human and rat TRPA1 channels respectively.

Alomone Labs

TRPA1 channels

AP-18 (#A-250) is a highly pure, synthetic, and biologically active compound.

A Reversible TRPA1 Channel Blocker

209.7

4-(4-Chlorophenyl)-3-methylbut-3-en-2-oxime

Synthetic

C11H12ClNO.

1-100 µM.

AP-18 has an IC50 of 3.1 and 4.5 μM for human and mouse TRPA1, respectively1.

Up to 200 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).

Up to 200 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99% (HPLC)

55224-94-7

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(NE)-N-[(Z)-4-(4-chlorophenyl)-3-methylbut-3-en-2-ylidene]hydroxylamine.