ANA-12 | Alomone Labs A-215 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ANA-12

catalog :

A-215

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Paris A, Hayer K, Oved J, Avgousti D, Toulmin S, Zepp J, et al. STAT3-BDNF-TrkB signalling promotes alveolar epithelial regeneration after lung injury. Nat Cell Biol. 2020;22:1197-1210 pubmed publisher

image

image 1 :

Alomone Labs BQ788 sodium saltinhibits ET-B receptor-mediated Ca2+mobilization in CHO cells. - Dose-response plot ofBQ788 sodium salt(#B-176) inhibition of ET-B receptor-mediated endothelin-1-evoked Ca2+mobilization (IC50= 8.23 nM). Cells were loaded with Calcium 6 dye incubated with BQ788 sodium salt and stimulated with 30 nM endothelin-1 (EC80). Changes in intracellular Ca2+levels were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?.

product information

CAT :

A-215

SKU :

A-215_10 mg

Product Name :

ANA-12

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

ANA-12 is a low molecular weight ligand that acts as a selective tropomyosin receptor kinase B (TrkB) antagonist. The compound shows direct binding to TrkB receptor and prevents its activation by BDNF with high potency, without altering TrkA and TrkC functions. The compound is considered to be a valuable tool for studying the BDNF-TrkB signaling pathway1,2.A study indicated that administration of ANA-12 has successfully reduced anxiety and depression related behaviors tested in mice1.TrkB receptor and BDNF are key mediators in the pathophysiology of several mood disorders, including drug addiction, anxiety and depression. They play an important role in the regulation of synaptic transmission and plasticity1.

Supplier :

Alomone Labs

Target :

TrkB receptor

Long Description :

ANA-12 (#A-215) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Selective Antagonist of TrkB Receptor

MW :

407.49

Source :

Synthetic

Molecular formula :

C22H21N3O3S.

Effective Concentration :

100-200 µM.

Activity :

ANA-12 is a selective TrkB antagonist, shown to bind directly and selectively to TrkB, preventing activation by BDNF and inhibiting processes downstream of TrkB, in a 2-site mode of action (IC50 values are 45.6 nM and 41.1 μM for the high and low affinity sites, respectively), without altering TrkA and TrkC functions. In adult mice ANA-12 decreases TrkB activity in the brain without affecting neuronal survival, and reduces anxiety and depression related behaviors1.

Reconstitution and Solubility :

Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

219766-25-3

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-[2-[(2-oxoazepan-3-yl)carbamoyl]phenyl]-1-benzothiophene-2-carboxamide.

more info or order :

Alomone Labs product webpage