A-889425 | Alomone Labs A-195 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

A-889425

catalog :

A-195

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Chen Y, Gao J, Li L, Sellitto C, Mathias R, Donaldson P, et al. The Ciliary Muscle and Zonules of Zinn Modulate Lens Intracellular Hydrostatic Pressure Through Transient Receptor Potential Vanilloid Channels. Invest Ophthalmol Vis Sci. 2019;60:4416-4424 pubmed publisher
Mandal A, Shahidullah M, Delamere N. TRPV1-dependent ERK1/2 activation in porcine lens epithelium. Exp Eye Res. 2018;172:128-136 pubmed publisher

image

image 1 :

Alomone Labs A-889425 inhibits capsaicin activated TRPV1 channels heterologously expressed in C-6 cells. - Cells were loaded with Fluo-3 AM and changes in intracellular Ca2+were measured. Normalized fluorescence before (control gray dotted line) and after application of 1Mcapsaicin(#C-125) (black lines) in cells pre-incubated without or with 0.5 MA-889425(#A-195) (top and bottom lines as indicated). 10 min pre-incubation with 0.5 M A-889425 abolishes capsaicin-evoked rise in intracellular Ca2+.

image 2 :

Expression of CASK in rat hypothalamus - Immunohistochemical staining of rat hypothalamus free floating frozen sections using Anti-CASKAntibody (#AIP-018) (1:200). CASK staining (green) appears in fibers with varicosities (arrows) running from the ventromedial hypothalamus (VMH) along the base of the brain. Nuclei are stained using DAPI (blue).

product information

CAT :

A-195

SKU :

A-195_1 mg

Product Name :

A-889425

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

TRPV1 (transient receptor potential vanilloid family type 1) channels are activated and/or modulated by noxious heat, capsaicin, protons and other endogenous agents released following tissue injury.A-889425 is a selective and competitive TRPV1 channel blocker with an IC50 of 335 nM to displace capsaicin at rat receptors, and has good penetration into the CNS1. In the somatic sensory system, receptor-selective TRPV1 antagonists, such as A-889425, attenuate mechanical hyperalgesia in models of inflammatory, osteoarthritic, and neuropathic pain2. Administration of A-889425 inhibited mechanically evoked activity of wide dynamic range (WDR) neurons in the rat spinal dorsal horn under conditions of peripheral inflammation but not in uninjured rats1.In addition to actions on the nociceptive system, several TRPV1 blockers like A-889425 have been reported to affect thermoregulation by transiently raising core body temperature following systemic administration in both animals and humans3.

Supplier :

Alomone Labs

Target :

TRPV1 channels

Long Description :

A-889425 (#A-195) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of TRPV1 Channels

MW :

425.4

Source :

Synthetic

Molecular formula :

C19H18F3N3O3S.

Effective Concentration :

0.5 nM - 50 µM.

Activity :

A-889425 blocks TRPV1 channels with an IC50 of 335 nM (rat) and 34 nM (human)1.

Reconstitution and Solubility :

DMSO up to 100 mM. Centrifuge all product preparations before use (10000 x g for 1 min).

Solubility :

DMSO up to 100 mM. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

1072921-02-8

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1-(3-methylpyridin-2-yl)-N-(4-(trifluoromethylsulfonyl)phenyl)-1,2,3,6-tetrahydropyridine-4-carboxamide.

more info or order :

Alomone Labs product webpage