product summary
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company name :
Alomone Labs
product type :
chemical
product name :
AM 404
catalog :
A-190
more info or order :
citations: 2
| Reference |
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image
image 1 :
Alomone Labs AM 404 activates TRPV1 channels heterologously expressed in C-6 cells. - Cells were loaded with Fluo-3AM and changes in intracellular Ca2+were measured.Normalized fluorescence before (control gray dotted line) and after application of 1 M and 2 MAM 404(#A-190) (black lines as indicated).
image 2 :
Western blot analysis of rat brain (lanes 1 and 4) mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell line lysates: - 12.Anti-CASKAntibody (#AIP-018) (1:400).3. Anti-CASK Antibody (1:200).456. Anti-CASK Antibody preincubated with the negative control antigen.
product information
CAT :
A-190
SKU :
A-190_10 mg
Product Name :
AM 404
Group Type :
Non Antibodies
Product Type :
S. Molecules
Storage After Reconstitution :
Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Product Page - Scientific background :
N-arachidonoylaminophenol (AM 404) belongs to a group of bioactive N-acylamines. It is a potent activator of TRPV1 (EC50 = 26 nM)1, and a CB1 cannabinoid receptor ligand2. Recent work on the mechanism of AM 404 has suggested that the inhibition of fatty acid amide hydrolase (FAAH) by AM 404 (IC50 = 6 μΜ) is likely responsible for all of its attributed "reuptake" properties, since intracellular FAAH hydrolysis of anandamide changes the intra/extracellular anandamide equilibrium3. AM 404 also inhibits cyclooxygenase COX-1 and COX-2, thus attenuating prostaglandin synthesis4. AM 404 produces analgesia in the mouse formalin and hot plate tests5 and potentiates the analgesic effect of anandamide in the mouse hot plate test6. Furthermore, AM 404 decreases the expression of c-fos in the spinal cord of neuropathic rats, an effect inhibited by both TRPV1 and cannabinoid receptor antagonists7.
Supplier :
Alomone Labs
Target :
TRPV1, CB1 receptors
Long Description :
AM 404 (#A-190) is a highly pure, synthetic, and biologically active compound.
Short Description :
An Activator of TRPV1 Channels and a Ligand of CB1 Cannabinoid Receptors
MW :
395.6
Synonyms :
N-arachidonoylaminophenol
Source :
Synthetic
Molecular formula :
C26H37NO2.
Effective Concentration :
0.5 nM - 50 µM.
Activity :
AM 404 activates TRPV1 channels with EC50 = 0.04 µM1, and serves as a ligand for CB1 cannabinoid receptors2.
Reconstitution and Solubility :
100 mM in DMSO, 50 mM in ethanol (dissolves slowly). Centrifuge all product preparations before use (10000 x g 5 min).
Solubility :
100 mM in DMSO, 50 mM in ethanol (dissolves slowly). Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions :
Up to four weeks at 4°C or three months at -20°C.
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Purity :
>98% (HPLC)
CAS No :
198022-70-7
Form :
Lyophilized powder.
Comment :
Contact Alomone Labs for technical support and product customization
UNSPSC :
41116134
Bioassay Tested :
Yes
Chemical Name :
N-(4-Hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide.
more info or order :
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel
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