AMG 9810 | Alomone Labs A-180 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

AMG 9810

catalog :

A-180

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Obi S, Nakajima T, Hasegawa T, Nakamura F, Sakuma M, Toyoda S, et al. Heat induces myogenic transcription factors of myoblast cells via transient receptor potential vanilloid 1 (Trpv1). FEBS Open Bio. 2019;9:101-113 pubmed publisher
Obi S, Nakajima T, Hasegawa T, Kikuchi H, Oguri G, Takahashi M, et al. Heat induces interleukin-6 in skeletal muscle cells via TRPV1/PKC/CREB pathways. J Appl Physiol (1985). 2017;122:683-694 pubmed publisher

image

image 1 :

Alomone Labs AMG 9810 inhibits Olvanil-activated TRPV1 channels heterologously expressed in C-6 cells - Cells were loaded with Fluo-3 AM and changes in intracellular Ca2+was measured. Normalized fluorescence before (control gray dotted line) and after application of 10 MOlvanil (#O-115) either without or after incubation with 1 M and 10 MAMG 9810(#A-180) black lines as indicated.

image 2 :

Peptide (C)KERDRYAYKIHLPE corresponding to amino acid residues 277-290 of mouse CASK (AccessionO70589). Intracellular.

product information

CAT :

A-180

SKU :

A-180_10 mg

Product Name :

AMG 9810

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

AMG 9810 (E)-3-(4-t-butylphenyl)-N-(2, 3- dihydrobenzo [b] [1,4] dioxin-6-yl) acrylamide is a potent, non-vanilloid, competitive antagonist of the vanilloid receptor 1 (TRPV1) with IC50 values for human TRPV1 of 24.5 nM and rat TRPV1 of 85.6 nM.Additionally, it has been shown to compete with other modes of TRPV1 activation, including protons (IC50s = 92.7 and 294 nM for human and rat, respectively), heat (IC50s = 15.8 and 21 nM for human and rat, respectively), and the endogenous ligands anandamide, N-arachidonoyl dopamine (IC50s = 8.5 and 260 nM for human and rat, respectively), and oleoyldopamine.Furthermore, AMG 9810 blocks capsaicin-induced calcitonin gene-related peptide (CGRP) release and capsaicin-evoked depolarization in rat dorsal root ganglia neurons.Finally, AMG 9810 was shown to be a potent and selective antagonist of TRPV1 that can significantly reverse thermal and mechanical hyperalgesia in an animal model of inflammatory pain1.

Supplier :

Alomone Labs

Target :

TRPV1 channels

Long Description :

AMG 9810 (#A-180) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of TRPV1 Channels

MW :

337.4

Source :

Synthetic

Molecular formula :

C21H23NO3.

Effective Concentration :

0.5 nM - 50 µM.

Activity :

AMG 9810 blocks TRPV1 with IC50 ~0.01 µM1.

Reconstitution and Solubility :

Ethanol 50 mM. Centrifuge all product preparations before use (10 000 x g for 1 min).

Solubility :

Ethanol 50 mM. Centrifuge all product preparations before use (10 000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>96% (HPLC)

CAS No :

545395-94-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(2E)-N-(2,3-Dihydro-1,4-benzodioxin-6-yl)-3-[4-(1,1-dimethylethyl)phenyl]-2-propenamide.

more info or order :

Alomone Labs product webpage