Aconitine | Alomone Labs A-150 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Aconitine

catalog :

A-150

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Perrier J, Rasmussen H, Jørgensen L, Berg R. Intense Activity of the Raphe Spinal Pathway Depresses Motor Activity via a Serotonin Dependent Mechanism. Front Neural Circuits. 2017;11:111 pubmed publisher
Ferreira F, Luxardi G, Reid B, Zhao M. Early bioelectric activities mediate redox-modulated regeneration. Development. 2016;143:4582-4594 pubmed
Aubert L, Guilbert M, Corbet C, GÃ©not E, Adriaenssens E, Chassat T, et al. NGF-induced TrkA/CD44 association is involved in tumor aggressiveness and resistance to lestaurtinib. Oncotarget. 2015;6:9807-19 pubmed
Schulz Klaus B, Lessmann V, Endres T. BDNF-dependent consolidation of fear memories in the perirhinal cortex. Front Behav Neurosci. 2013;7:205 pubmed publisher

image

image 1 :

Immuno-colocalization of GIRK2 (Kir3.2) and LRRK2 in mouse substantia nigra pars compacta - Immunohistochemical staining of immersion-fixed free floating mouse brain frozen sections using Guinea pig Anti-GIRK2 (Kir3.2) Antibody(#AGP-013) (1:200) and rabbitAnti-LRRK2 Antibody(#ANR-102) (1:200). A. Kir3.2 staining (red) appears in profiles of dopaminergic neurons (arrows). B. LRRK2 staining (green) is detected in several cell bodies in the SNC. C. Merge of the two images demonstrates colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

A-150

SKU :

A-150_100 mg

Product Name :

Aconitine

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are present in most excitable cell membranes and play an important role in generating action potentials. A variety of toxins and chemicals are known to either block or modulate Na+ channels and have proven to be invaluable in investigating the physiological characteristics of these channels1. Most notably, tetrodotoxin (TTX), a potent and selective blocker of Na+ channels, binds to neurotoxin receptor site 1 to block them2.Aconitine is a diterpense two-ester alkaloid derived from the tubers of aconitinum plants and has been used as traditional herbal medicine in China and Japan since ancient times. Modern pharmacological studies have shown that aconitine has effects of analgesia and anesthesia, anti-inflammatory, immune regulation, anti-tumor and cardiotonic actions3,4. Concurrently, aconitine also presents enormous toxicity5. It is well known for its toxic effects on the heart and central nervous system where it causes cardiac arrhythmias, systemic paralysis and coma6. Aconitine alters the kinetics and voltage dependence of Na+ channel activation, inhibits Na+ channel inactivation through binding to neurotoxin receptor site 2 and causes persistent activation of Na+ channels at the resting membrane potential7.

Supplier :

Alomone Labs

Target :

NaV Na+ channels

Long Description :

Aconitine (#A-150) is a highly pure, natural, and biologically active compound.

Short Description :

An Activator of NaV Channels

MW :

645.74

Synonyms :

Acetylbenzoylaconine

Source :

Natural

Molecular formula :

C34H47NO11.

Effective Concentration :

100 nM - 1 μM.

Activity :

Aconitine modulates voltage-dependent sodium channels in a complex way1. It activates the channel at low voltages, while at high voltages its effects appear inhibitory.

Reconstitution and Solubility :

DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

302-27-2

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(1α,3α,6α,14α,15α,16β)-20-Ethyl-1,6,16-trimethoxy-4-(methoxymethyl)aconitane-3,8,13,14,15-pentol 8-acetate 14-benzoate.

more info or order :

Alomone Labs product webpage