domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 42
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 42
Western blot analysis of rat brain membranes: - 1. Anti-TRPC6 Antibody (#ACC-017), (1:200).2. Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).
Expression of TRPC6 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-TRPC6 Antibody (#ACC-017). A. TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti parvalbumin in the same brain section. C. Confocal merge of TRPC6 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Expression of TRPC6 in human kidney from MN patients - Immunohistochemical staining of human biopsy specimens from MN patients FSGS patients and normal kidney tissue from tumor nephrectomy samples using Anti-TRPC6 Antibody (#ACC-017). TRPC6 expression was below detection levels in control kidneys and only slightly induced in FSGS glomeruli whereas MN kidneys mostly showed moderate to strong positivity for TRPC6.Adapted from Kistler A.D. et al. (2013) J. Biol. Chem. 288 36598. with permission of the American Society for Biochemistry and Molecular Biology.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 14
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 14
Western blot analysis of rat brain lysates: - 1. Anti-TRPC6 (extracellular) Antibody (#ACC-120), (1:200).2. Anti-TRPC6 (extracellular) Antibody, preincubated with TRPC6 (extracellular) Blocking Peptide (#BLP-CC120).
Expression of TRPC6 in rat PC12 cells - Cell surface detection ofTRPC6 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC6 (extracellular)Antibody (#ACC-120) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining with DAPI as the counterstain. C. Merged image of A and B.
TRPC6 is required for stretch activation in myocytes. - Electrophysiological recordings of myocytes incubated with Anti-TRPC6 (extracellular) Antibody (#ACC-120) in the absence (upper left) and in the presence (lower left) of the control peptide antigen. Immunocytochemical staining of living rat cerebral artery smooth muscle cells using Anti-TRPC6 (extracellular) antibody. Preincubation with the control peptide antigen blocks TRPC6 immunostaining (lower right panel).Adapted from Gonzales A.L. et al. with permission of the American Association for the Advancement of Science.
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guinea-pigs polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of rat brain membranes: - 1. Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP), (1:200).2. Guinea pig Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).
Expression of TRPC6 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP). A. TRPC6 (green) appears in the soma of Purkinje cells (white arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates intense expression of TRPC6 in the soma of Purkinje cells and lower expression in the molecular (Mol) layer. DAPI is used as the counterstain (blue).
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
Cell surface detection of TRPC6 by direct flow cytometry in live intact mouse P815 mastocytoma cells: Cell surface detection of TRPC6 by direct flow cytometry in live intact mouse P815 mastocytoma cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-TRPC6 (extracellular)-FITC Antibody (#ACC-120-F), (5µg).
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