domestic rabbit polyclonal (NA)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 16
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 16
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 2) and human HL-60 acute promyelocytic leukemia cell line lysate (lanes 3 and 4): - 1,3. Anti-Human Orai1 (extracellular) Antibody (#ACC-060), (1:200). 2,4. Anti-Human Orai1 (extracellular) Antibody, preincubated with Human Orai1 (extracellular) Blocking Peptide (#BLP-CC060).
Peptide (C)KKQPGQPRPTSK corresponding to amino acid residues 203-214 of human Orai1 (AccessionQ96D31). 2ndextracellular loop.
Cell surface detection of Orai1 in intact HL-60 (A)and Jurkat cells (B). - ___Unstained cells.___Cells +Anti-Human Orai1 (extracellular)Antibody (#ACC-060) (5-10 g antibody/0.5-1 x 106cells).
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mouse monoclonal (3F11/D10/B9.)
reactivity: human
application: western blot, immunocytochemistry
citations: 6
reactivity: human
application: western blot, immunocytochemistry
citations: 6
Western blot analysis using Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025), (1:200): - 1. HEK-293 cells transfected with human Orai1.2. HEK-293 cells transfected with control vector.
Cell surface detection of Orai1 in intact living Jurkat cells: - ___Unstained cells.___Cells + MouseAnti-Human Orai1 (extracellular)Antibody (#ALM-025) (5-10 g antibody/0.5-1 x 106cells).
Expression of Orai1 in HEK-293 transfected cells - Cell surface detection ofOrai1 in intact living HEK-293 cells expressing Orai1. A. Extracellular staining of HEK-293 cells transfected with Orai1 using MouseAnti-Human Orai1 (extracellular)Antibody (#ALM-025) (red). B. Cells transfected with the empty vector show no Orai1 staining.
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mouse monoclonal (3F11/D10/B9.)
reactivity: human
conjugate: biotin
application: western blot, flow cytometry
citations: 2
reactivity: human
conjugate: biotin
application: western blot, flow cytometry
citations: 2
Western blot analysis using Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (1:500): - 1. Jurkat cells.2. HEK cells transfected with human Orai1.3. HEK cells transfected with control vector.
Cell surface detection of Orai1 in intact living Jurkat cells: Cell surface detection of Orai1 in intact living Jurkat cells: Black line: Unstained cells. Green line: Cells + Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (5-10 µg antibody/0.5-1 x 106 cells).
Alomone Labs L-689560 inhibits NMDA receptor channels expressed in Xenopus oocytes. - A. Representative time course of NR1/NR2A currents elicited by transient stimulations with 100 M glutamate + 100 M glycine every 50 sec while membrane potential was held at -60 mV. 4 min applications of 5 nM and 10 nM L-689560 (#L-225) reversibly inhibit current amplitude. B. Superimposed current traces from the recording shown in A following application of control 5 nM and 10 nM L-689560 (as indicated). Transient stimulation is indicated by the top horizontal bar.
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mouse monoclonal (3F11/D10/B9.)
reactivity: human
application: flow cytometry
citations: 2
reactivity: human
application: flow cytometry
citations: 2
Cell surface detection of Orai1 in intact living Jurkat cells: Cell surface detection of Orai1 in intact living Jurkat cells: Black line: Unstained cells. Green line: Cells + Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody (#ALM-025-FR), (5 µg antibody/0.5-1 x 106 cells).
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domestic rabbit polyclonal (NA)
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 1
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 1
Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells: Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells: Black line: Unstained cells. Red line: Cells + Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F), (10 µg/1x106 cells).
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