Alomone Labs TAK-044 inhibits Endothelin-B receptor-mediated Ca2+ mobilization in CHO cells. - Cells were loaded with Calcium-6 dye incubated with different concentrations ofTAK-044(#SPE-320) and stimulated by 30 nM Endothelin-1 (EC80). Changes in intracellular Ca2+were monitored as changes in relative fluorescence (RLU) using FLIPRTETRA. IC50was determined as 0.66 M.
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Alomone Labs Ro-51 inhibits P2X3 channels expressed in HEK-293 cells. - Dose-response curve of P2X3 inhibition byRo-51(#R-195). Cells were loaded with Calcium-6 dye incubated for 5 min with increasing concentrations of Ro-51 and stimulated by 3 M ??-MeATP. Changes in intracellular Ca2+following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?. IC50was determined at 4 nM.
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Alomone Labs Evans Blue tetrasodium salt reversibly inhibits AMPA receptors heterologously expressed inXenopusoocytes. - Inhibitory effect of 20 MEvans Blue tetrasodium salt(#E-110) on GluR1 currents elicited at -80 mV holding potential by continuous stimulation with 10 M glutamate (indicated by uppermost horizontal bar).
Western blot analysis of mouse brain membranes: - 1.Guinea pigAnti-KCNMA1 (KCa1.1) (1097?1096) Antibody (#AGP-014) (1:200).2. Guinea pig Anti-KCNMA1 (KCa1.1) (1097?1096) Antibody preincubated with the negative control antigen.
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Alomone Labs NS-11021 activatesKCa1.1 channels expressed inXenopusoocytes. - A. Time course of normalized KCa1.1 currents at +50 mV. Currents were elicited by 150 ms voltage ramp from holding potential of -100 mV to +100 mV every 10 sec.NS-11021(#N-335) applied at 2 M and 10 M as indicated (bars) reversibly enhanced KCa1.1 currents. B. Current traces upon application of control or of 2 M and 10 M NS-11021 (as indicated).
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