Anti-TRPV4 Antibody
Alomone Labs
catalog: ACC-034

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 69

Western blot analysis of rat brain lysates: - 1. Anti-TRPV4 Antibody (#ACC-034), (1:200).2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).

Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 Antibody (#ACC-034). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.

Expression of TRPV4 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.A D. Staining usingAnti-TRPV4Antibody (#ACC-034) (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.B E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. Merged image of panels A and B.F. Merged image of panels D and E.
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Anti-TRPV4 (extracellular) Antibody
Alomone Labs
catalog: ACC-124

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 10

Western blot analysis of rat brain lysate: - 1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).2. Anti-TRPV4 (extracellular) Antibody, preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).

Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.

Expression of TRPV4 in human U-87 cells - Cell surface detection ofTRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular)Antibody (#ACC-124) (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.
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Anti-TRPV4-ATTO Fluor-550 Antibody
Alomone Labs
catalog: ACC-034-AO

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: immunohistochemistry, immunocytochemistry
citations: 5

Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections with Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO), (1:20). TRPV4 is present in DRG neurons (red). Note that staining is stronger in small cells (arrows). Hoechst 33342 is used as the counterstain.
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Guinea pig Anti-TRPV4 Antibody
Alomone Labs
catalog: Acc-034-GP

guinea-pigs polyclonal (NA)
reactivity: mouse, rat
application: western blot

Western blot analysis of rat brain membranes: - 1. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:1500). 2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
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Anti-TRPV4 (extracellular)-FITC Antibody
Alomone Labs
catalog: ACC-124-F

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry

Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells: Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells: Black line: Cells.   Red line: Cells + rabbit IgG isotype control-FITC.   Green line: Cells + Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F), (2.5 µg).
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Anti-TRPV4 (extracellular) Antibody- PE Antibody
Alomone Labs
catalog: ACC-124-PE

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: flow cytometry

Cell surface detection of TRPV4 by direct flow cytometry in live intact mouse J774 macrophage cells:

Cell surface detection of TRPV4 by direct flow cytometry in live intact human U-87 MG glioblastoma cell s :

Cell surface multicolor flow cytometry analysis of live intact mouse J774 macrophage cells with TRPV4 and Piezo1:
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