domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 26
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 26
Western blot analysis with Anti-BDNF Antibody: - 1,5. Recombinant human BDNF protein (#B-250).2,6. Recombinant mouse proBDNF protein (#B-240).3,7. Recombinant human beta-NGF protein (#N-245).4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).Lanes 5-8: Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010). Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
Expression of BDNF in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-BDNF Antibody (#ANT-010). A. BDNF (red) appears in Purkinje cells (upward pointing arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (downward pointing arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein (green) in the same section demonstrates the distribution of BDNF to neuronal as well as to astrocytic cellular components. C. Confocal merge of BDNF and GFAP.
Peptide (C)GEFGGHDKPKITAWE corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (AccessionO35458). Cytoplasmic N-terminus.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 14
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 14
Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: - 1,2. Anti-proBDNF Antibody (#ANT-006), (1:200).3,4. Anti-proBDNF Antibody, preincubated with proBDNF Blocking Peptide (#BLP-NT006).
Expression of proBDNF in rat cerebellum - Immunohistochemical staining of rat cerebellum with Anti-proBDNF Antibody (#ANT-006). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.
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guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
Western blot analysis of 100 ng of each Recombinant human BDNF protein (#B-250) (lanes 1 and 5), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 6), Recombinant human Neurotrophin-3 (NT-3) protein (#N-260) (lanes 3 and 7) and Recombinant human beta-NGF protein (#N-245) (lanes 4 and 8): - 1-4. Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:500).5-8. Guinea pig Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010).Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
Expression of BDNF in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:400), followed by goat anti-guinea pig-Cy3. BDNF staining (red) in the hippocampal dentate gyrus region, appears in neuronal profiles in the granule layer (G) and in interneurons in the hilus (H) region (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
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guinea-pigs polyclonal (NA)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2
Western blot analysis of 50 ng of each Recombinant human Val66Met proBDNF (cleavage resistant) protein (#B-456) (lanes 1 and 4), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 5) and Recombinant human proBDNF protein (#B-257) (lanes 3 and 6): - 1-3. Guinea pig Anti-proBDNF Antibody (#ANT-006-GP), (1:200).4-6. Guinea pig Anti-proBDNF Antibody, preincubated with proBDNF Blocking Peptide (#BLP-NT006).
Expression of proBDNF in rat spinal cord - Immunohistochemical staining of immersion-fixed, free floating rat spinal cord frozen sections using Guinea pig Anti-proBDNF Antibody (#ANT-006-GP), (1:1000). proBDNF (red) is detected in the superficial layer (arrow) of the dorsal horn of the spinal cord. The myelinated columns are devoid of staining. Cell nuclei are stained with DAPI (blue).
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry, flow cytometry
citations: 1
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry, flow cytometry
citations: 1
Expression of proBDNF in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-proBDNF-ATTO-488 Antibody (#ANT-006-AG), (1:50), (green). Staining is present in DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Peptide (C)KDFLNYTLEERLED corresponding to amino acid residues 39-52 of rat Glucose Transporter 3 (AccessionQ07647). 1stextracellular loop.
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