citations: 1
Alomone Labs DM-PPP inhibits mGluR1 mediated Ca2+mobilization in U2OS cells. - Dose response of normalized inhibition of human mGluR1 mediated L-Glutamate evoked Ca2+mobilization byDM-PPP(#D-255). IC50was determined at 144 nM. hmGluR1-expressing cells were loaded with Ca2+-sensitive dye incubated with different concentrations of DM-PPP and stimulated by 15 M L-Glutamate (EC80). Changes in intracellular Ca2+following stimulation were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?.
quantity: 10 µg, 25 µg, 50 µg, 0.1 mg
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Alomone Labs Okadaic Acid induces apoptosis in Jurkat cells. - Cells were grown to 70% confluency followed by the addition of 1MOkadaic Acid (ammonium salt)(#O-800) (orOkadaic Acid (sodium salt)(#O-900)) or vehicle for 6 h. At the end of the incubation cell proteins were resolved by SDS-PAGE and probed with anti-Caspase 3 antibody.
Alomone Labs Okadaic Acid inhibits PP2A in 3T3-L1 cells. - Cells were grown to 70% confluence and serum starved for 18 h. Forin vivophosphatase activity the growth medium was replaced with HENK?s and the cells were preincubated in the presence or absence of 100 nMOkadaic Acid (ammonium salt)(#O-800) (orOkadaic Acid (sodium salt)(#O-900)) and 100 nM1-Norokadaone(#N-750) followed by a 20 min incubation with or without 20 M NEM. The cells were then stimulated with 5 ng/ml PDGF for 30 min. At the end of the incubation cell proteins were resolved by SDS-PAGE and probed with anti-phospho-Akt antibodies.
Western blot analysis ofrat brain synaptosomal fraction(lanes 1 and 3) and mousebrain synaptosomal fraction(lanes 2 and 4): - 12. Guinea pigAnti-D1Dopamine ReceptorAntibody (#AGP-100) (1:400).34. Guinea pig Anti-D1Dopamine Receptor Antibody preincubated with the negative control antigen.
quantity: 10 µg, 25 µg, 5 x 25 µg, 0.1 mg
price:
to the supplier
