Anti-P2X4 Receptor Antibody
Alomone Labs
catalog: APR-002

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation
citations: 28

Western blot analysis of rat brain membranes: - 1. Anti-P2X4 Receptor Antibody (#APR-002), (1:200).2. Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).

Expression of P2RX4 in rat brain  - Immunohistochemical staining of rat brain red nucleus using Anti-P2X4 Receptor Antibody (#APR-002). A. P2RX4 (green) appears in fibers surrounding cell shapes (arrows). B. Calbindin 28K (red) appears in large neurons. C. Merge of P2RX4 and Calbindin 28K suggests variable density of P2RX4 expressing fibers on red nucleus neurons. DAPI is used as the counterstain (blue).

Expression of P2X4 receptor in lysosomes. - Immunocytochemical staining of fixed and permeated COS1 cells using Anti-P2X4 Receptor Antibody (#APR-002). A. P2X4 staining (green) co-localizes with Lamp1 (red) a lysosomal marker. B. Fractionation of COS1 cells shows that P2X4 receptor is detected in the lysosome fraction along with Lamp1. C. Similar results are obtained when P2X4 is heterologously expressed.Adapted from Huang P. et al. (2014) with permission of The American Society for Biochemistry and Molecular Biology.
quantity:
price:
to the supplier

Anti-P2X4 Receptor (extracellular) Antibody
Alomone Labs
catalog: APR-024

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 6

Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 rat brain glioma cell line lysate (lanes 3 and 7) and SH-SY5Y human brain neuroblastoma cell line lysate (lanes 4 and 8): - 1-4. Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (1:200).5-8. Anti-P2X4 Receptor (extracellular) Antibody, preincubated with P2X4 Receptor (extracellular) Blocking Peptide (#BLP-PR024).

Peptide (C)RDLAGKEQRTLTK corresponding to amino acid residues 301-313 of rat P2X4 receptor (AccessionP51577). Extracellular.

Expression of P2RX4 in rat pheochromocytoma cell line (PC12) - Cell surface detection ofP2RX4 in intact living PC12 cells. A. Extracellular staining of cells withAnti-P2X4 (extracellular) Antibody (#APR-024) (red 1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.
quantity:
price:
to the supplier

Guinea pig Anti-P2X4 Receptor Antibody
Alomone Labs
catalog: APR-002-GP

guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot
citations: 2

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates: - 1,2. Guinea pig Anti-P2X4 Receptor Antibody (#APR-002-GP), (1:500).3.    Guinea pig Anti-P2X4 Receptor Antibody (1:1000).4-6. Guinea pig Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).
quantity:
price:
to the supplier

Anti-P2X4 Receptor (extracellular)-FITC Antibody
Alomone Labs
catalog: APR-024-F

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
citations: 1

Cell surface detection of P2RX4 in live intact mouse BV-2 microglia cells: Cell surface detection of P2RX4 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-P2X4 Receptor (extracellular)-FITC Antibody (#APR-024-F), (2.5 µg).
quantity:
price:
to the supplier

Anti-P2X4 Receptor (extracellular)-PE Antibody
Alomone Labs
catalog: APR-024-PE

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: PE
application: flow cytometry
citations: 1

Cell surface detection of P2X4 Receptor by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of P2X4 Receptor by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-PE. Green line: Cells + Anti-P2X4 Receptor (extracellular)-PE Antibody (#APR-024-PE), (2.5µg).
quantity:
price:
to the supplier

Anti-P2X4 Receptor (extracellular)-APC Antibody
Alomone Labs
catalog: APR-024-APC

domestic rabbit polyclonal
reactivity: human, mouse, rat

Cell surface detection of P2X4 by direct flow cytometry in live intact mouse BV-2 microglia cells:

Cell surface detection of P2X4 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:

Cell surface detection of P2X4 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
quantity:
price:
to the supplier