domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 12
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 12
Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).
Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone usingAnti-NGFAntibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 10
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 10
Western blot analysis of Recombinant mouse proNGF protein (#N-250), (lanes 1-3) and Recombinant human proNGF protein (#N-280), (lanes 4-6): - 1,4. 100 ng + Anti-proNGF Antibody (ANT-005), (1:200).2,5. 250 ng + Anti-proNGF Antibody (1:200).3,6. 500 ng + Anti-proNGF Antibody (1:200).
Expression of proNGF in rat brain sections - Immunohistochemical staining of proNGF in rat brain sections using Anti-proNGF Antibody (#ANT-005). A. Staining of astrocytes was performed using mouse anti-glial fibrillary acidic protein (GFAP) (red). B. Co-localization between GFAP and proNGF (orange). C. Staining of proNGF (green).
Western blot analysisof mouse submandibular glands (SMGs): - 1.Anti-proNGF Antibody (#ANT-005)(1:200).2.Anti-proNGF Antibody preincubated with the control peptide antigen.
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mouse monoclonal (HAL06.)
reactivity: human, mouse, rat
citations: 6
reactivity: human, mouse, rat
citations: 6
Indirect ELISA of adsorbed proNGF and NGF - Increasing amounts ofRecombinant mouse proNGF protein(#N-250) (blue) andNative mouse NGF 2.5S protein (99%) (#N-240) (red) were adsorbed to a microplate and detected with MouseNGF/proNGF Neutralizing Antibody(#ALM-006) (2 ?g/ml) followed by goat-anti-mouse-HRP and chromogenic substrate.
Indirect ELISA of adsorbed neurotrophic factors - Neurotrophic factorsNative mouse NGF 2.5S protein (99%)(#N-240)Native mouse NGF 7S protein(#N-130)Recombinant human beta-NGF protein(#N-245)Recombinant human proNGF protein(#N-280)Recombinant human BDNF protein(#B-250)Recombinant human Neurotrophin-3 (NT-3) protein(#N-260) andRecombinant human Neurotrophin-4 (NT-4) protein(#N-270) all at 2 ?g/ml were adsorbed to a microplate and analyzed using Mouse NGF/proNGF Neutralizing Antibody(#ALM-006) (2 ?g/ml) followed by goat-anti-mouse-HRP and chromogenic substrate.
Neutralization of NGF-mediated PC12 proliferation - PC12 cells were grown in the absence of serum and in the presence ofNative mouse NGF 2.5S protein (99%)(#N-240) (50 ng/ml) as indicated. Varying concentrations of MouseNGF/proNGF Neutralizing Antibody(#ALM-006) were added to the culture and cell viability was established after 24 h using the XTT method. Note that 600 ng/ml Mouse NGF/proNGF Neutralizing Antibody was able to completely block the activity of 50 ng/ml mouse NGF.
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guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
Western blot analysis of 100 ng Recombinant mouse proNGF protein (#N-250) (lanes 1 and 3) and Recombinant human proNGF protein (#N-280) (lanes 2 and 4): - 1-2. Guinea pig Anti-proNGF Antibody (#ANT-005-GP), (1:200).3-4. Guinea pig Anti-proNGF Antibody, preincubated with the blocking peptide.
Expression of proNGF in mouse cortex - Immunohistochemical staining of perfusion-fixed mouse brain frozen sections using Guinea pig Anti-proNGF Antibody (#ANT-005-GP), (1:400), followed by goat-anti-guinea pig-Cy3. proNGF staining (red) appears in the pyramidal layer (arrows). Nuclei were stained with DAPI (blue).
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