citations: 44
citations: 2
Alomone Labs Apamin-Biotin blocks rat SK2 channels stably transfected in HEK293T cells. - A. Time course ofApamin-Biotin(#STA-200-B) action on SK2 channels. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and cells were stimulated by a 150 ms voltage ramp from -120 mV to +60 mV every 10 sec. 1 nM 10 nM and 50 nM Apamin-Biotin were perfused during 120 sec as indicated by the bar at 0 mV. B. Superimposed examples of SK2 channel current in the absence (control black) and presence of 1 nM (green) 10 nM (red) and 50 nM (blue) Apamin-Biotin (taken from the experiment in A).
quantity:
price:
to the supplier
citations: 1
Alomone Labs M8-B hydrochloride inhibits TRPM8 channels expressed in HEK-293 cells. - Dose response curve of TRPM8 inhibition byM8-B hydrochloride(#M-220). Cells were loaded with Calcium-5 dye incubated for 10 min with increasing concentrations of M8-B hydrochloride and activated by 30 nM Icilin. Changes in intracellular Ca2+following agonist application were detected as changes in 485nm/525nm fluorescence (RFU). IC50was determined at 20 nM.
Alomone Labs CyPPA enhances hSK2 channel currents in transiently transfected HEK293T cells. - Cells were stimulated with 100 ?MCyPPA(#C-110) and currents were measured using whole cell voltage-clamp. A. Time course at -20mV showing the effect of 100 ?M CyPPA and its inhibition with 150 nMApamin (#STA-200). B. Representative currents at control conditions (bottom) and following application of 100 ?M CyPPA (top). Currents were elicited by a 150 ms voltage ramp from -120 to 60 mV every 10 seconds from a holding potential of -80 mV (current responses are shown between -120 and -10 mV). Leaks were subtracted offline.
quantity:
price:
to the supplier