Anti-KV1.3 (KCNA3) Antibody
Alomone Labs
catalog: APC-002

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 13

Western blot analysis of rat brain membranes: - 1. Anti-KV1.3 (KCNA3) Antibody (#APC-002), (1:200).2. Anti-KV1.3 (KCNA3) Antibody, preincubated with Kv1.3/KCNA3 Blocking Peptide (#BLP-PC002).

Western blot analysisof rat brain membranes: - 1.Anti-KV1.3(KCNA3)Antibody (#APC-002) (1:200).2. Anti-KV1.3 (KCNA3) Antibody preincubated with the negative control antigen.

Cell surface detection of EAAT1 inlive intact human MEG-01 megakaryoblastic leukemia cells: - ___Cells.___Cells + Rabbit IgG isotype control-FITC.___Cells + Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody(#AGC-021-F) (5 g).
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Anti-KV1.3 (KCNA3) (extracellular) Antibody
Alomone Labs
catalog: APC-101

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 10

Western blot analysis of rat brain membranes:   - 1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

Peptide KDYPASTSQDSFEA(C) corresponding to amino acid residues 263-276 of human KV1.3 (AccessionP22001). 1stextracellular loop.
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Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody
Alomone Labs
catalog: APC-101-GP

guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 4

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

Expression of KV1.3 in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .
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Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
Alomone Labs
catalog: APC-101-B

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 1

Western blot analysis of human Jurkat T cell leukemia cell lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1-2. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B), (1:1000).3-4. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

Multiplex staining of KV1.3 and KV1.5 in mouse cerebellum - Immunohistochemical staining of mouse perfusion-fixed frozen brain sections using Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B) (1:400), and Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody (#APC-004-AO), (1:60). A. KV1.3 staining (green) is detected in Bergmann glia soma and processes in the molecular layer (Mol) (arrow). B. Same section shows staining for KV1.5 (red). C. Merge of the two images suggests considerable co-localization in the soma of Bergmann glia. In the molecular layer, the distribution of KV1.5 is diffuse, unlike the discrete staining for KV1.3 in glial processes. Cell nuclei were visualized with DAPI (blue).

Cell surface detection of KV1.3 in live intact human Jurkat T cell leukemia cell line: Cell surface detection of KV1.3 in live intact human Jurkat T cell leukemia cell line: Black line: Cells + Streptavidin-Alexa647. Black line: Cells + Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B), (1:10) + Streptavidin-Alexa647.
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Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody
Alomone Labs
catalog: APC-101-F

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: immunocytochemistry, flow cytometry
citations: 1

Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells: Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells: Red line: Unstained cells. Green line: Cells + Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F), (0.5-5 µg antibody / 1x106 cells).
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Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
Alomone Labs
catalog: APC-101-PE

domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: PE
application: flow cytometry

Cell surface detection of KV1.3 in human Jurkat T-cell leukemia cells: Cell surface detection of KV1.3 in human Jurkat T-cell leukemia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-PE. Green line: Cells + Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody (#APC-101-PE), (5 µg).
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