domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 57
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 57
Western blot analysis of rat brain membranes: - 1. Anti-Kir4.1 (KCNJ10) Antibody (#APC-035), (1:400).2. Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).
Peptide (C)KLEESLREQAEKEGSALSVR corresponding to amino acid residues 356-375 of rat Kir4.1 (AccessionP49655). Intracellular C-terminus.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
citations: 8
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
citations: 8
Expression of Kir4.1 in rat cerebellum - Immunohistochemical staining of frozen sections of rat cerebellum using Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody (#APC-035-AG) (green), (1:50). Staining is specific for Bergmann glial cells prolongations (white arrows) in the molecular layer (ML) and astrocytes (yellow arrows) in the granular layer (GL). Purkinje cell bodies are stained with fluorescent Nissl stain (red). Hoechst 33342 (blue) is used as counterstain.
Western blot analysis of rat heart membrane (lanes 1 and 3) rat spleen lysate (lanes 2 and 4) mouse heart membrane (lanes 5 and 6) and human Colo205 cell linelysate(lanes 7 and 8): - 1257. Guinea pig Anti-KCNQ1 Antibody (#AGP-050) (1:200).3468. Guinea pig Anti-KCNQ1 Antibody preincubated with the negative control antigen.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165).
Peptide (C)HGDLLELGPPANHT corresponding to amino acid residues 93-106 of rat Kir4.1 (AccessionP49655). Extracellular loop.
Expression of Kir4.1 in human U-87 MG cells - Cell surface detection ofKir4.1 in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells usingAnti-Kir4.1(KCNJ10)(extracellular) Antibody (#APC-165) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
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guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): - 1-3. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:800).4-6. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).
Expression of Kir4.1 in rat hippocampal dentate gyrus - Immunohistochemical staining of rat frozen brain sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:300). A. Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer (Mol). B. The same section was stained with mouse anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C. Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like” cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.
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