citations: 5
Alomone Labs WRW4 blocks Ca2+transients in differentiated HL-60 cells generated by MMK1 application. - Cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following application (indicated by arrow) of 1MMMK1(#GPM-120). 30min pre-incubation with various concentrations ofWRW4(#GPW-110) resulted in a dose-dependent inhibition of FPR2 activation. A. Normalized fluorescence levels before and after application (indicated by an arrow) of control saline solution (black) or of 1M MMK1 in cells pre-incubated with 0 M (red) 0.25 M (green) 1 M (purple) and 5 M (blue) WRW4. B. Inhibition of FPR2 activation plotted against WRW4 concentrations.
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Alomone Labs MMK1 evokes a concentration-dependent rise in intracellular Ca2+in non-differentiated HL-60 cells. - Non-differentiated HL-60 cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following agonist application. Application of MMK1 evoked a concentration-dependent rise in intracellular Ca2+. Normalized fluorescence before and after application (arrow) of control and of 1 nM 10 nM 100 nM and 1 M MMK1 (as indicated).
Expression of Glucose Transporter 2 in rat cerebellum and hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GLUT2 (SLC2A2)Antibody (#AGT-022) (1:120) followed by goat-anti-rabbit-AlexaFluor-488 (green). Cell nuclei are stained with DAPI (blue). A. Staining in cerebellum sections reveals GLUT2 labeling in Purkinje cells (arrows). B. Staining in hippocampus dentate gyrus shows labeling of both granule cells (horizontal arrow) and in hilar interneurons (vertical arrows).
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Alomone Labs WKYMVM activates Ca2+ transients in differentiated HL-60 cells. - Cells (1.3% DMSO day 6) were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following application (indicated by arrow) of 1 ?MWKYMVM(#GPW-100) (green) compared to control (black saline perfusion).
Expression of BDNF in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections usingGuinea pigAnti-BDNFAntibody (#AGP-021) (1:400) followed by goat anti-guinea pig-Cy3. BDNF staining (red) in the hippocampal dentate gyrus region appears in neuronal profiles in the granule layer (G) and in interneurons in the hilus (H) region (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
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Alomone Labs WKYMVm activates Ca2+transients in HL-60 cells. - Non-differentiated HL-60 cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following application ofWKYMVm(#GPW-105) at different concentrations.Normalized fluorescence before and after application (indicated by arrow) of 1 nM 10 nM and 100 nM WKYMVm are shown compared to control (black).
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Alomone LabsLosartan potassiuminhibits the activation of AT1R expressed in CHO-K1-mt aequorin-G?16 cells. - Dose-response curve for the inhibition of AT1R expressed in CHO-K1-mt aequorin-G?16 cells. Ca2+response as detected by elevation in aequorin derived fluorescence following 0.04 nM Angiotensin II application was inhibited by increasing concentrations ofLosartan potassium(#L-185). 20 nM Losartan potassium fully inhibited the activation by Angiotensin II.
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