domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 30
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 30
Western blot analysis of rat brain lysates: - 1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).
Expression of GABRA1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Distribution of GABRA1 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABRA1 is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
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domestic rabbit polyclonal (NA)
reactivity: mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
citations: 1
reactivity: mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
citations: 1
Multiplex staining of CaV1.2 and GABA(A) α1 Receptor in rat and mouse hippocampus - Immunohistochemical staining of mouse and rat hippocampal dentate gyrus using Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG) in the same section. Both CaV1.2 (red) and GABRA1 (green) are detected in neuron-shaped cells (arrows). Staining suggests partial colocalization between CaV1.2 and GABRA1 in a sub-population of rat dentate gyrus neurons. A. Mouse hippocampus. B. Rat hippocampus.
Immuno-colocalization of CaV1.2 and GABA(A)?1 Receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pigAnti-CaV1.2(CACNA1C)Antibody (#AGP-001) andAnti-GABA(A)?1 Receptor (extracellular)-ATTO-488 Antibody(#AGA-001-AG).A. CaV1.2 (red) is detected mostly in Purkinje cells (arrow). B. In the same sectionGABA(A) ?1 Receptor (green) is observed in the granule layer. C. Merge of the two images suggests some colocalization between CaV1.2 and GABA(A) ?1 Receptor in the rat granule layer but only CaV1.2 appears in Purkinje cells.
Expression of GABRA1 in rat brain - Immunohistochemical staining of rat dentate gyrus usingAnti-GABA(A) ?1 Receptor (extracellular)-ATTO-488Antibody(#AGA-001-AG) (green). Staining reveals hilar neurons (arrows) and fibers coursing through the granule layer (G).DAPI is used as the counterstain(blue).
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guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate: - 1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400).4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001). Following a broad screen of secondary antibodies, the following were used for this application:Western blot analysis:#106-035-006 (Jackson ImmunoResearch) #A7289 (Sigma)
Multiplex staining of KCC2 and GABA(A) α1 Receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using rabbit Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:400) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400). A. KCC2 immunoreactivity (green) is detected in both the granule layer (GL) and the molecular layer (MOL). B. GABA(A) α1 receptor staining (red) is observed mainly in the granule layer (GL). C. Merge of the two images demonstrates colocalization in the granule layer (arrows). Cell nuclei are stained with DAPI (blue).
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: flow cytometry
reactivity: human, mouse, rat
application: flow cytometry
Cell surface detection of GABRA1 by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of GABRA1 by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody (#AGA-001-F), (5µg).
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