domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 5
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 5
Western blot analysis of rat brain membranes: - 1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500).2. Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Expression of mGluR5 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).
Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells: - ___ Cells.___ Cells + goat-anti-rabbit-FITC.___ Cells +Anti-mGluR5 (extracellular) Antibody(#AGC-007) (5 g) + goat-anti-rabbit-FITC.
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guinea-pigs polyclonal (NA)
reactivity: mouse, rat
application: western blot
citations: 1
reactivity: mouse, rat
application: western blot
citations: 1
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGC-007-GP), (1:400).3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007). Following a broad screen of secondary antibodies, the following were used for this application:#106-035-006 (Jackson ImmunoResearch) #A7289 (Sigma)
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domestic rabbit polyclonal (NA)
reactivity: mouse, rat
conjugate: ATTO 594
application: immunohistochemistry, immunocytochemistry
reactivity: mouse, rat
conjugate: ATTO 594
application: immunohistochemistry, immunocytochemistry
Expression of mGluR5 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:50). A. mGluR5 (red) is detected in pyramidal layer neurons (arrow). B. DAPI is used to visualize cell nuclei (blue). C. Merge of A and B.
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domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells: Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-mGluR5 (extracellular)-FITC Antibody (#AGC-007-F), (5 µg).
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