This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
TNFa Tumor necrosis factor alpha
catalog :
IMS01-102-339
quantity :
100 µl
price :
348 USD
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human
product information
Product number :
IMS01-102-339
Product name :
TNFa Tumor necrosis factor alpha
Quantity :
100 µl
List Price :
348 USD
Immunogen :
Purified full length recombinant protein P01375
Host :
Chicken
Clonality :
Polyclonal
Purity :
Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.
Format :
Liquid
Recommended dilutions :
1 : 5000 (WB)
Expected apparent MW :
26 kDa
Confirmed reactivity :
Human
Predicted reactivity :
Cat, Dog, Horse, Pig, Primates
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots.ELISA performance: Purified antigen is precoated on the microtitre plate. IMS01-102-339 is used as primary antibody at various dilutions. ALP-conjugated anti-chicken IgG is used as a secondary antibody at a dilution of 1:1000.
Background :
Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Storage :
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation
UniProt number :
P01375
Commodity Code :
38220000
Our Category :
Cancer
Research area :
Cancer Research
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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